PMID- 14678230
OWN - NLM
STAT- MEDLINE
DCOM- 20041207
LR  - 20190827
IS  - 0305-1870 (Print)
IS  - 0305-1870 (Linking)
VI  - 30
IP  - 12
DP  - 2003 Dec
TI  - Phytosterol additives increase blood pressure and promote stroke onset in 
      salt-loaded stroke-prone spontaneously hypertensive rats.
PG  - 919-24
AB  - 1. To assess the effect of dietary phytosterol on stroke and the lifespan of 
      salt-loaded stroke-prone spontaneously hypertensive rats (SHRSP), we investigated 
      the effects of the addition of phytosterol to soybean oil (phytosterol content: 
      0.3%) on stroke onset, lifespan following onset of stroke and overall lifespan 
      compared with canola oil (phytosterol content: 0.9%). 2. Six-week-old male SHRSP 
      were fed a test diet prepared by the addition of canola oil (CA diet), soybean 
      oil (SO diet), soybean oil plus 0.6% phytosterol (SO + 0.06P diet) or soybean oil 
      plus 4.5% phytosterol (SO + 0.45P diet) as a 10% fat source. 3. Systolic blood 
      pressure (SBP) increased in the SO + 0.06P and SO + 0.45P groups compared with 
      the SO group and the increase was dependent on the amount of phytosterol added, 
      indicating that the addition of phytosterol to soybean oil may promote an 
      increase in SBP in salt-loaded SHRSP. 4. The onset of stroke was shortest in the 
      SO + 0.45P group and survival after the onset of stroke was shortest in the CA 
      group. Consequently, the SO + 0.45P and CA groups showed marked lifespan 
      shortening, indicating that a fivefold greater amount of phytosterol was required 
      to produce an effect equivalent to that of canola oil. 5. Investigation of the 
      mRNA expression of ATP-binding cassette (ABC) transporters involved in intestinal 
      phytosterol absorption indicated significant decreases in the intestinal mRNA 
      expression of Abcg5 and Abcg8 in SHRSP and Wistar-Kyoto rats compared with Wistar 
      rats. 6. In conclusion, the addition of phytosterol to soybean oil elevated SBP 
      and promoted the onset of stroke, which may cause a reduction in survival time. 
      However, a fivefold greater amount of phytosterol was required to produce an 
      effect that was equivalent to the survival time-shortening effect of canola oil. 
      The significant decrease in the intestinal mRNA expression of Abcg5 and Abcg8 in 
      SHRSP may be responsible, at least in part, for the unfavourable effects observed 
      following the addition of phytosterol.
FAU - Ogawa, Hiroshi
AU  - Ogawa H
AD  - Department of Hygiene and Division of Basic Medical Science, Kinki University 
      School of Medicine, Osaka-Sayama City, Osaka, Japan. ogawa-h@med.kindai.ac.jp
FAU - Yamamoto, Kazuo
AU  - Yamamoto K
FAU - Kamisako, Toshinori
AU  - Kamisako T
FAU - Meguro, Tadamichi
AU  - Meguro T
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Australia
TA  - Clin Exp Pharmacol Physiol
JT  - Clinical and experimental pharmacology & physiology
JID - 0425076
RN  - 0 (ATP-Binding Cassette Transporters)
RN  - 0 (Fat Substitutes)
RN  - 0 (Fatty Acids, Monounsaturated)
RN  - 0 (Phytosterols)
RN  - 0 (RNA, Messenger)
RN  - 0 (Rapeseed Oil)
RN  - 0 (Sodium Chloride, Dietary)
RN  - 8001-22-7 (Soybean Oil)
SB  - IM
MH  - ATP-Binding Cassette Transporters/drug effects/genetics
MH  - Animals
MH  - Dose-Response Relationship, Drug
MH  - Eating/physiology
MH  - Fat Substitutes/administration & dosage/adverse effects/analysis
MH  - Fatty Acids, Monounsaturated/administration & dosage/adverse 
      effects/pharmacokinetics
MH  - Gene Expression/drug effects/genetics
MH  - Hypertension/*chemically induced/complications
MH  - Intestines/anatomy & histology/chemistry/drug effects
MH  - Male
MH  - Phytosterols/administration & dosage/*adverse effects/analysis
MH  - RNA, Messenger/chemistry/drug effects/genetics
MH  - Rapeseed Oil
MH  - Rats
MH  - Rats, Inbred SHR
MH  - Rats, Inbred WKY
MH  - Rats, Wistar
MH  - Sodium Chloride, Dietary/pharmacology
MH  - Soybean Oil/administration & dosage/chemistry/pharmacokinetics
MH  - Stroke/*chemically induced/complications/mortality
EDAT- 2003/12/18 05:00
MHDA- 2004/12/16 09:00
CRDT- 2003/12/18 05:00
PHST- 2003/12/18 05:00 [pubmed]
PHST- 2004/12/16 09:00 [medline]
PHST- 2003/12/18 05:00 [entrez]
AID - 10.1111/j.1440-1681.2003.03939.x [doi]
PST - ppublish
SO  - Clin Exp Pharmacol Physiol. 2003 Dec;30(12):919-24. doi: 
      10.1111/j.1440-1681.2003.03939.x.