PMID- 14680704
OWN - NLM
STAT- MEDLINE
DCOM- 20040223
LR  - 20181130
IS  - 0378-1097 (Print)
IS  - 0378-1097 (Linking)
VI  - 229
IP  - 2
DP  - 2003 Dec 12
TI  - Branching of o-nitrobenzoate degradation pathway in Arthrobacter protophormiae 
      RKJ100: identification of new intermediates.
PG  - 231-6
AB  - We have earlier reported a novel reductive pathway for o-nitrobenzoate (ONB) 
      degradation (at 0.5 mM) in Arthrobacter protophormiae RKJ100, which proceeds via 
      the formation of o-hydroxylaminobenzoate (HABA) and anthranilate (AA). During 
      growth of this organism at 40 times higher concentration (20 mM) of ONB, 
      3-hydroxyanthranilate (HAA) was identified as an intermediate by thin layer 
      chromatography, gas chromatography and high performance liquid chromatography 
      studies. Crude cell extracts of ONB-grown cells showed HAA 3,4-dioxygenase 
      activity suggesting HAA as a terminal aromatic intermediate of the catabolic 
      energy-yielding pathway as shown before in Pseudomonas fluorescens strain KU-7. 
      HAA is further cleaved to 2-amino-3-carboxymuconic-6-semialdehyde by the action 
      of HAA 3,4-dioxygenase. In this report we propose that ONB degradation occurs via 
      the formation of HABA and the pathway branches at this point to form the two 
      different aromatic intermediates AA and HAA by the action of a reductase and a 
      mutase, respectively.
FAU - Pandey, Gunjan
AU  - Pandey G
AD  - Institute of Microbial Technology, Sector 39-A, Chandigarh 160036, India.
FAU - Paul, Debarati
AU  - Paul D
FAU - Jain, Rakesh K
AU  - Jain RK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - FEMS Microbiol Lett
JT  - FEMS microbiology letters
JID - 7705721
RN  - 0 (Azo Compounds)
RN  - 0 (Nitrobenzoates)
RN  - 1634-82-8 (HABA)
RN  - 1UQB1BT4OT (3-Hydroxyanthranilic Acid)
RN  - EC 1.- (Oxidoreductases)
RN  - EC 5.4.- (Intramolecular Transferases)
SB  - IM
MH  - 3-Hydroxyanthranilic Acid/metabolism
MH  - Arthrobacter/*metabolism
MH  - Azo Compounds/metabolism
MH  - *Biodegradation, Environmental
MH  - Intramolecular Transferases/metabolism
MH  - Nitrobenzoates/*metabolism
MH  - Oxidoreductases/metabolism
MH  - Substrate Specificity
EDAT- 2003/12/19 05:00
MHDA- 2004/02/24 05:00
CRDT- 2003/12/19 05:00
PHST- 2003/12/19 05:00 [pubmed]
PHST- 2004/02/24 05:00 [medline]
PHST- 2003/12/19 05:00 [entrez]
AID - S0378109703008449 [pii]
AID - 10.1016/S0378-1097(03)00844-9 [doi]
PST - ppublish
SO  - FEMS Microbiol Lett. 2003 Dec 12;229(2):231-6. doi: 
      10.1016/S0378-1097(03)00844-9.