PMID- 14681197
OWN - NLM
STAT- MEDLINE
DCOM- 20041102
LR  - 20171116
IS  - 0006-3363 (Print)
IS  - 0006-3363 (Linking)
VI  - 70
IP  - 4
DP  - 2004 Apr
TI  - Lineage, maturity, and phenotype of uterine murine dendritic cells throughout 
      gestation indicate a protective role in maintaining pregnancy.
PG  - 1018-23
AB  - Dendritic cells (DCs) are known to play a major role in the induction, 
      maintenance, and regulation of immune responses. Recently, DCs have been 
      described to be present at the feto-maternal interface in human decidua. However, 
      only limited information is available about DC presence, phenotype, and--more 
      importantly--function throughout gestation. Thus, we analyzed local (uterine) and 
      systemic (blood) DCs in a murine model. DBA/2J mated CBA/J females with vaginal 
      plugs were separated and killed on Gestation Days (GDs) 1.5, 3.5, 5.5, 6.5, 7.5, 
      8.5, 10.5, 13.5, 15.5, or 17.5. Frequency of uterine and blood CD11c+ DC, 
      phenotype (coexpression of CD8alpha and major histocompatibility complex class II 
      [MHC II] antigens), and presence of intracellular cytokines (interleukins 12 and 
      10) were determined by flow cytometry. The morphology of DC in the pregnant 
      uterus was evaluated by immunohistochemistry. In uterus, the relative number of 
      CD11c+ cells increased from GD 5.5, reaching a plateau on GD 9.5 until GD 17.5, 
      while a transient peak of systemic CD11c+ cells was found on GD 8.5 and 10.5. The 
      vast majority of uterine DCs were CD8alpha- and thus, belonged to the myeloid 
      lineage. Interestingly, a significant peak of lymphoid DC was present on GD 1.5 
      and 5.5. Further, significantly more intracellular interleukin 10 than 
      interleukin 12 was present in CD11c+ cells. Interestingly, mature DCs (MHC II+) 
      were diminished from GD 5.5 to 8.5. Characterization of CD11c+ cell kinetics in 
      uterus and blood reveals variation of phenotype during pregnancy, pointing toward 
      an eminent immunoregulatory role of DCs throughout gestation at the feto-maternal 
      interface.
FAU - Blois, Sandra M
AU  - Blois SM
AD  - Charite, Department of Internal Medicine, Biomedizinisches Forschungszentrum, 
      Campus Virchow, Humboldt University of Berlin, 13353 Berlin, Germany.
FAU - Alba Soto, Catalina D
AU  - Alba Soto CD
FAU - Tometten, Mareike
AU  - Tometten M
FAU - Klapp, Burghard F
AU  - Klapp BF
FAU - Margni, Ricardo A
AU  - Margni RA
FAU - Arck, Petra C
AU  - Arck PC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20031217
PL  - United States
TA  - Biol Reprod
JT  - Biology of reproduction
JID - 0207224
RN  - 0 (CD11c Antigen)
RN  - 0 (Histocompatibility Antigens Class II)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/cytology
MH  - CD11c Antigen/blood/metabolism
MH  - Cell Line
MH  - Cellular Senescence
MH  - Dendritic Cells/*cytology/metabolism/*physiology
MH  - Female
MH  - Histocompatibility Antigens Class II/metabolism
MH  - Lymphoid Tissue/cytology
MH  - Mice
MH  - Mice, Inbred CBA
MH  - Mice, Inbred DBA
MH  - Phenotype
MH  - Pregnancy
MH  - Pregnancy, Animal/metabolism/*physiology
MH  - Tissue Distribution
MH  - Uterus/*cytology/metabolism
EDAT- 2003/12/19 05:00
MHDA- 2004/11/04 09:00
CRDT- 2003/12/19 05:00
PHST- 2003/12/19 05:00 [pubmed]
PHST- 2004/11/04 09:00 [medline]
PHST- 2003/12/19 05:00 [entrez]
AID - biolreprod.103.022640 [pii]
AID - 10.1095/biolreprod.103.022640 [doi]
PST - ppublish
SO  - Biol Reprod. 2004 Apr;70(4):1018-23. doi: 10.1095/biolreprod.103.022640. Epub 
      2003 Dec 17.