PMID- 14682236
OWN - NLM
STAT- MEDLINE
DCOM- 20040128
LR  - 20061115
IS  - 0379-4172 (Print)
IS  - 0379-4172 (Linking)
VI  - 30
IP  - 8
DP  - 2003 Aug
TI  - Chromosomal location and expression of green fluorescent protein (gfp) gene in 
      microspore derived transgenic barley (Hordeum vulgare L.).
PG  - 697-705
AB  - Four doubled haploid barley lines (A, C, D, E) derived from gfp (green 
      fluorescent protein) transformation and selection following particle bombardment 
      of microspores were studied for gene expression pattern and the location of 
      genome inserts. The integration sites were detected by fluorescence in situ 
      hybridization (FISH) using the gfp plasmid DNA as a probe. Plants from events A, 
      C, D and E all have a single insert site on chromosome 7L(5HL) at different 
      locations while line E has a second insert site on chromosome 5S(7HS). All 
      original transgenic plants were hemizygous for the transgenes and segregated in 
      the T1 and T2 generations. Although line D had no GFP expression, FISH and PCR 
      could detect gfp gene on its chromosome in transformed plants. Expression levels 
      of GFP varied with lines and tissues examined. Plants from line C showed good 
      expression in pollen and an intermediate level in root tips. Plants from A have 
      intermediate expression of GFP in the pollen and light expression in the root 
      tips. Line E showed strong expression in the root-tips and an intermediate level 
      of GFP in the pollen. Lines A and C segregated as a single Mendelian locus while 
      E segregated in a duplicate loci ratio (15:1) on seedling root tips but had low 
      expression frequency in the pollen. PCR results were consistent with GFP 
      expression on root tips in the three segregating lines. The expression of GFP for 
      lines D and E was abnormal and may be related to the physical location of the 
      transgene or the gene construct used.
FAU - Chen, Jian-Min
AU  - Chen JM
AD  - College of Agriculture, Nanjing Agricultural University, Nanjing 210095, China. 
      Jmchen@yzu.edu.cn
FAU - Carlson, A R
AU  - Carlson AR
FAU - Wan, Jian-Min
AU  - Wan JM
FAU - Kasha, K J
AU  - Kasha KJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Yi Chuan Xue Bao
JT  - Yi chuan xue bao = Acta genetica Sinica
JID - 7900784
RN  - 0 (DNA, Plant)
RN  - 0 (Luminescent Proteins)
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
MH  - Chromosome Mapping
MH  - Chromosomes, Plant/*genetics
MH  - DNA, Plant/genetics
MH  - Gene Expression Regulation, Plant
MH  - Green Fluorescent Proteins
MH  - Hordeum/*genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Luminescent Proteins/*genetics/metabolism
MH  - Microscopy, Fluorescence
MH  - Plant Roots/genetics
MH  - Plants, Genetically Modified
MH  - Pollen/*genetics/growth & development
MH  - Polymerase Chain Reaction
EDAT- 2003/12/20 05:00
MHDA- 2004/01/30 05:00
CRDT- 2003/12/20 05:00
PHST- 2003/12/20 05:00 [pubmed]
PHST- 2004/01/30 05:00 [medline]
PHST- 2003/12/20 05:00 [entrez]
PST - ppublish
SO  - Yi Chuan Xue Bao. 2003 Aug;30(8):697-705.