PMID- 14686112
OWN - NLM
STAT- MEDLINE
DCOM- 20040106
LR  - 20041117
IS  - 0367-8326 (Print)
IS  - 0367-8326 (Linking)
VI  - 39
IP  - 3-4
DP  - 2002 Sep-Dec
TI  - Anti-neutrophil cytoplasmic antibodies (ANCA) in malaria.
PG  - 51-9
AB  - Various autoantibodies like anti-nuclear antibodies (ANA), anti-double stranded 
      DNA (anti-dsDNA), anti-histone antibodies (AHA), anti-neutrophil cytoplasmic 
      antibodies (ANCA), anti-myeloperoxidase (anti-MPO), anti-proteinase3 (anti-PR3) 
      and anti-lactoferrin (anti-LF) antibodies were studied in 173 acute hospitalised 
      patients suffering from malaria of which 160 patients had P. falciparum and 
      remaining 13 had P. vivax infection. Standard methods like indirect 
      immunofluorescence (IIF) microscopy along with Confocal microscopy and ELISA were 
      used for identifying and quantifying the autoantibodies and IIF patterns on PMN 
      and HL-60 cells were studied for ANCA classification. Also HEp-2 cells were used 
      for ANA detection, while estimation of anti-dsDNA, AHA, anti-MPO, anti-PR3 and 
      anti-LF were tested using ELISA. Sera from malaria patients showed prominent 
      immunofluorescence staining patterns where 23.8% cases had ANA in P. falciparum 
      group as compared to 15.4% in P. vivax group and ANCA was found to be present in 
      20% in P. falciparum and 15.4% in P. vivax group. An interesting observation was 
      that, of the total ANCA positives, 59% had p-ANCA, 5.9% had c-ANCA and 44.1% of 
      the cases showed the 'atypical' or X-ANCA pattern. When p-ANCA positivity was 
      compared with c-ANCA positivity among these patients, a good statistical 
      correlation was noted with OR = 16, chi 2 = 16.43, EF = 0.46 and p-value = 5.037E 
      0.5. ELISA showed 31.2% anti-MPO and 6.2% anti-PR3 in P. falciparum cases while 
      the two ANCA positive cases in P. vivax had anti-MPO. Anti-LF was found to be 
      present in 40.6% cases. Neither the P. falciparum nor P. vivax contained 
      autoantibodies with specificities similar to the characteristic lupus 
      autoantibodies such as double stranded DNA (dsDNA). ANCA positivity develops in 
      some types of malarial infection also with the presence of various autoantibodies 
      which is important from a clinical point of view and should be carefully 
      evaluated in those geographic areas where malaria is endemic. It also alerts us 
      to the fact, whether in cases of repeated malarial infections in susceptible 
      individuals, vasculitic disorders, which through ANCA pathways develop, could 
      lead to renal and other complications.
FAU - Pradhan, Vandana
AU  - Pradhan V
AD  - Institute of Immunohaematology (ICMR), K.E.M. Hospital, Parel, Mumbai-400 012, 
      India.
FAU - Badakere, S S
AU  - Badakere SS
FAU - Shankarkumar, U
AU  - Shankarkumar U
FAU - Iyer, Y S
AU  - Iyer YS
FAU - Ghosh, K
AU  - Ghosh K
FAU - Karnad, D
AU  - Karnad D
LA  - eng
PT  - Journal Article
PL  - India
TA  - Indian J Malariol
JT  - Indian journal of malariology
JID - 17540010R
RN  - 0 (Antibodies, Antineutrophil Cytoplasmic)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Age Distribution
MH  - Aged
MH  - Antibodies, Antineutrophil Cytoplasmic/*isolation & purification
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Humans
MH  - Malaria, Falciparum/classification/*immunology
MH  - Malaria, Vivax/classification/*immunology
MH  - Middle Aged
EDAT- 2003/12/23 05:00
MHDA- 2004/01/07 05:00
CRDT- 2003/12/23 05:00
PHST- 2003/12/23 05:00 [pubmed]
PHST- 2004/01/07 05:00 [medline]
PHST- 2003/12/23 05:00 [entrez]
PST - ppublish
SO  - Indian J Malariol. 2002 Sep-Dec;39(3-4):51-9.