PMID- 14687559
OWN - NLM
STAT- MEDLINE
DCOM- 20040413
LR  - 20191108
IS  - 0882-4010 (Print)
IS  - 0882-4010 (Linking)
VI  - 36
IP  - 2
DP  - 2004 Feb
TI  - Mycobacterium avium-superoxide dismutase binds to epithelial cell aldolase, 
      glyceraldehyde-3-phosphate dehydrogenase and cyclophilin A.
PG  - 67-74
AB  - Mycobacterium avium complex (MAC) adheres, invades and multiplies inside 
      epithelial cells. Earlier, we demonstrated two MAC protein adhesins, 25 and 31 
      kDa, binding with HEp-2 cells. The 25 kDa MAC adhesin was found to be superoxide 
      dismutase (SOD). In this study, epithelial cell (HEp-2 and A549) ligands for 
      MAC-SOD were identified by probing two-dimensional western blots of epithelial 
      extracts with MAC proteins followed by monoclonal anti-MAC-SOD antibodies. Three 
      epithelial cell proteins with molecular masses 43, 40 and 18 kDa, present in both 
      membrane and cytosolic fractions, were found to bind with MAC-SOD. Based on the 
      N-terminal amino acid sequences, the 43, 40 and 18 kDa epithelial proteins were 
      identified as aldolase, glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and 
      cyclophilin A (CypA), respectively. Furthermore, MAC-SOD was found to bind to 
      purified rabbit muscle aldolase, GAPDH and recombinant CypA in western blotting.
FAU - Reddy, Venkata M
AU  - Reddy VM
AD  - Department of Biomedical Sciences, University of Illinois College of Medicine, 
      Rockford, IL 61107, USA. vmreddy@uic.edu
FAU - Suleman, Funmilayo G
AU  - Suleman FG
LA  - eng
GR  - AI-39419/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Microb Pathog
JT  - Microbial pathogenesis
JID - 8606191
RN  - 0 (Adhesins, Bacterial)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - EC 1.2.1.- (Glyceraldehyde-3-Phosphate Dehydrogenases)
RN  - EC 4.1.2.13 (Fructose-Bisphosphate Aldolase)
RN  - EC 5.2.1.- (Cyclophilin A)
SB  - IM
MH  - Adhesins, Bacterial/chemistry
MH  - Blotting, Western
MH  - Cell Line
MH  - Cyclophilin A/chemistry/*metabolism
MH  - Electrophoresis, Gel, Two-Dimensional
MH  - Epithelial Cells/*enzymology/ultrastructure
MH  - Fructose-Bisphosphate Aldolase/chemistry/*metabolism
MH  - Glyceraldehyde-3-Phosphate Dehydrogenases/chemistry/*metabolism
MH  - Humans
MH  - Molecular Weight
MH  - Mycobacterium avium Complex/*enzymology
MH  - Protein Binding
MH  - Superoxide Dismutase/chemistry/*metabolism
EDAT- 2003/12/23 05:00
MHDA- 2004/04/14 05:00
CRDT- 2003/12/23 05:00
PHST- 2003/12/23 05:00 [pubmed]
PHST- 2004/04/14 05:00 [medline]
PHST- 2003/12/23 05:00 [entrez]
AID - S0882401003001840 [pii]
AID - 10.1016/j.micpath.2003.09.005 [doi]
PST - ppublish
SO  - Microb Pathog. 2004 Feb;36(2):67-74. doi: 10.1016/j.micpath.2003.09.005.