PMID- 14687582
OWN - NLM
STAT- MEDLINE
DCOM- 20040930
LR  - 20191108
IS  - 1043-4666 (Print)
IS  - 1043-4666 (Linking)
VI  - 25
IP  - 1
DP  - 2004 Jan 7
TI  - The effect of tumor necrosis factor-alpha on D-fructose intestinal transport in 
      rabbits.
PG  - 21-30
AB  - Tumor necrosis factor-alpha (TNF-alpha) is an important immunoregulatory cytokine 
      involved in septic responses during bacterial infection. The aim of this study 
      was to examine the effect of TNF-alpha on the transport of D-fructose across 
      rabbit jejunum. A sepsis condition was evoked by intravenous administration of 
      this cytokine and hematological and plasma parameters were analyzed and body 
      temperature was recorded. D-Fructose transport was assayed in rabbit jejunum. 
      Sugar absorption in TNF-alpha treated rabbits was lower than in control animals. 
      TNF-alpha decreased both the mucosal-to-serosal transepithelial flux and the 
      transport across brush border membrane vesicles of D-fructose. The number of 
      D-fructose transporters (GLUT5) was analyzed by Western blot in an attempt to 
      explain this inhibition. TNF-alpha treated animals had lower levels of GLUT5, 
      indicating a reduction in the expression of GLUT5 protein and therefore in 
      transport capacity. The inhibition could also be related with the secretagogue 
      effect of TNF-alpha on the gut since the intracellular tissue water was affected 
      and the absence of chloride ion in the incubation medium partly removed the 
      cytokine inhibition on sugar intestinal transport in treated rabbits. Finally, in 
      terms of possible mediators involved in the TNF-alpha effect, nitric oxide and 
      prostaglandins appeared to play a role in the inhibition of D-fructose intestinal 
      uptake.
FAU - Garcia-Herrera, J
AU  - Garcia-Herrera J
AD  - Department of Pharmacology and Physiology, Veterinary Faculty, University of 
      Zaragoza, Miguel Servet 177, 50013 Zaragoza, Spain.
FAU - Navarro, M A
AU  - Navarro MA
FAU - Marca, M C
AU  - Marca MC
FAU - de la Osada, J
AU  - de la Osada J
FAU - Rodriguez-Yoldi, M J
AU  - Rodriguez-Yoldi MJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Cytokine
JT  - Cytokine
JID - 9005353
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Glucose Transporter Type 5)
RN  - 0 (Monosaccharide Transport Proteins)
RN  - 0 (Prostaglandins)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 30237-26-4 (Fructose)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - V55S2QJN2X (NG-Nitroarginine Methyl Ester)
RN  - XXE1CET956 (Indomethacin)
SB  - IM
MH  - Animals
MH  - Biological Transport/drug effects
MH  - Enterocytes/drug effects/metabolism/ultrastructure
MH  - Enzyme Inhibitors/pharmacology
MH  - Fructose/*metabolism
MH  - Glucose Transporter Type 5
MH  - Indomethacin/pharmacology
MH  - Intestinal Absorption/*drug effects
MH  - Intestinal Mucosa/drug effects/*metabolism
MH  - Intestines/drug effects
MH  - Male
MH  - Microvilli/drug effects/metabolism
MH  - Monosaccharide Transport Proteins/drug effects/metabolism
MH  - NG-Nitroarginine Methyl Ester/pharmacology
MH  - Nitric Oxide/metabolism
MH  - Prostaglandins/metabolism
MH  - Rabbits
MH  - Sepsis/metabolism
MH  - Tumor Necrosis Factor-alpha/*pharmacology
EDAT- 2003/12/23 05:00
MHDA- 2004/10/01 05:00
CRDT- 2003/12/23 05:00
PHST- 2003/12/23 05:00 [pubmed]
PHST- 2004/10/01 05:00 [medline]
PHST- 2003/12/23 05:00 [entrez]
AID - S104346660300320X [pii]
AID - 10.1016/j.cyto.2003.09.005 [doi]
PST - ppublish
SO  - Cytokine. 2004 Jan 7;25(1):21-30. doi: 10.1016/j.cyto.2003.09.005.