PMID- 14687611
OWN - NLM
STAT- MEDLINE
DCOM- 20040422
LR  - 20190901
IS  - 0197-0186 (Print)
IS  - 0197-0186 (Linking)
VI  - 44
IP  - 6
DP  - 2004 May
TI  - Involvement of histone phosphorylation in apoptosis of human astrocytes after 
      exposure to saline solution.
PG  - 459-67
AB  - We have previously found using inhibitors of protein phosphatase that 
      phosphorylation of histones may be involved in thymocyte apoptosis. In this 
      study, we examined whether histone modification occurs in astrocyte apoptosis 
      induced by a pathological condition in the absence of drug. Incubation of 
      cultured human astrocytes with growth medium for 24 h after exposure to saline 
      solution for 30 min induced an increase in terminal deoxynucleotidyl 
      transferase-mediated dUTP nick end labeling (TUNEL)-positive cells and nuclear 
      condensation, biochemical and morphological hallmarks of apoptotic cell death. 
      Acetic acid-urea-Triton X-100 (AUT) gel electrophoresis of the nuclear histone 
      fraction and N-terminal peptide analysis showed that the treatment with saline 
      solution caused rapid changes in phosphorylation of H2A subfamilies, but not in 
      histone acetylation. The phosphorylation of the two subtypes increased markedly, 
      whereas the phosphorylation of one subtype decreased. In contrast, exposure to 
      ACF-95, an artificial cerebrospinal fluid (CSF), was associated with little 
      induction of apoptotic cell death and induced less changes in histone 
      phosphorylation. These results support the previous idea that chemical 
      modification of histones is involved in the DNA fragmentation in astrocytes 
      undergoing apoptosis.
FAU - Enomoto, Riyo
AU  - Enomoto R
AD  - Department of Pharmacology, Faculty of Pharmaceutical Sciences, Kobe Gakuin 
      University, Ikawadani-cho, Nishi-ku, Kobe 651-2180, Japan.
FAU - Tatsuoka, Hiroyuki
AU  - Tatsuoka H
FAU - Komai, Tomoe
AU  - Komai T
FAU - Sugahara, Chiyoko
AU  - Sugahara C
FAU - Takemura, Kazu
AU  - Takemura K
FAU - Yamauchi, Aiko
AU  - Yamauchi A
FAU - Nishimura, Masuhiro
AU  - Nishimura M
FAU - Naito, Shinsaku
AU  - Naito S
FAU - Matsuda, Toshio
AU  - Matsuda T
FAU - Lee, Eibai
AU  - Lee E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Neurochem Int
JT  - Neurochemistry international
JID - 8006959
RN  - 0 (Histones)
RN  - 451W47IQ8X (Sodium Chloride)
SB  - IM
MH  - Amino Acid Sequence
MH  - *Apoptosis
MH  - Astrocytes/cytology/*metabolism
MH  - Histones/chemistry/*metabolism
MH  - Humans
MH  - In Situ Nick-End Labeling
MH  - Molecular Sequence Data
MH  - Phosphorylation
MH  - *Sodium Chloride
EDAT- 2003/12/23 05:00
MHDA- 2004/04/23 05:00
CRDT- 2003/12/23 05:00
PHST- 2003/12/23 05:00 [pubmed]
PHST- 2004/04/23 05:00 [medline]
PHST- 2003/12/23 05:00 [entrez]
AID - S019701860300175X [pii]
AID - 10.1016/s0197-0186(03)00175-x [doi]
PST - ppublish
SO  - Neurochem Int. 2004 May;44(6):459-67. doi: 10.1016/s0197-0186(03)00175-x.