PMID- 14687728
OWN - NLM
STAT- MEDLINE
DCOM- 20040402
LR  - 20190827
IS  - 0165-2478 (Print)
IS  - 0165-2478 (Linking)
VI  - 90
IP  - 2-3
DP  - 2003 Dec 15
TI  - Lipopolysaccharide preferentially induces 4-1BB ligand expression on human 
      monocyte-derived dendritic cells.
PG  - 215-21
AB  - Dendritic cells (DCs) represent a promising tool for immunotherapy. A key feature 
      in their action is to provide co-stimulatory signals for full activation of T 
      cells. In view of recent studies demonstrating the critical role of 4-1BB 
      co-stimulation in T cell response, it is of importance to optimize 4-1BB ligand 
      (4-1BBL) expression on human monocyte-derived DCs (MDDCs), the DC source of many 
      clinical studies. In this study, two types of MDDCs, generated in 
      granulocyte-macrophage colony-stimulating factor and interleukin-4 
      (GM-CSF/IL-4-DCs) or in interferon-beta and IL-3 (IFN-beta/IL-3-DCs), were 
      analyzed for 4-1BBL expression in response to several known DC activators. 
      Immature MDDCs expressed 4-1BBLs at very low levels. Lipopolysaccharide (LPS) was 
      the only activator that preferentially triggered 4-1BBL expression on either 
      MDDCs, but 4-1BBL-positive cells were significantly more frequently observed on 
      LPS-activated GM-CSF/IL-4-DCs (30.2+/-2.6% versus 14.3+/-1.2%). Combinations of 
      multiple activating signals did not bring about enhanced 4-1BBL stimulatory 
      capacity. In addition, plasmid DNA transfection and necrotic cell pulsing of 
      GM-CSF/IL-4-DCs for antigen loading also resulted in 4-1BBL up-regulation. 
      However, in all circumstances, the induced 4-1BBL levels were low in comparison 
      with CD80 co-stimulatory molecule. Finally, by demonstrating LPS-matured 
      GM-CSF/IL-4-DCs from sorted 4-1BBL(high) population augmented T cell expansion 
      and survival, we propose that efforts are required to increase 4-1BBL levels on 
      MDDCs achieved by current activation schemes.
FAU - Lee, Pao-Kung
AU  - Lee PK
AD  - Department of Microbiology, Soochow University, Wai Shuang Hsi, Shih Lin, Taipei, 
      11102, Taiwan, ROC.
FAU - Chang, Chun-Jung
AU  - Chang CJ
FAU - Lin, Chun-Ming
AU  - Lin CM
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Immunol Lett
JT  - Immunology letters
JID - 7910006
RN  - 0 (4-1BB Ligand)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (TNFSF9 protein, human)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 207137-56-2 (Interleukin-4)
RN  - 77238-31-4 (Interferon-beta)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - 4-1BB Ligand
MH  - Cell Differentiation/drug effects
MH  - Cell Division
MH  - Cells, Cultured
MH  - Dendritic Cells/cytology/*drug effects/immunology/*metabolism
MH  - Flow Cytometry
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/pharmacology
MH  - Humans
MH  - Interferon-beta/immunology
MH  - Interleukin-4/pharmacology
MH  - Lipopolysaccharides/*pharmacology
MH  - Monocytes/cytology/drug effects/metabolism
MH  - Time Factors
MH  - Tumor Necrosis Factor-alpha/*metabolism
EDAT- 2003/12/23 05:00
MHDA- 2004/04/03 05:00
CRDT- 2003/12/23 05:00
PHST- 2003/12/23 05:00 [pubmed]
PHST- 2004/04/03 05:00 [medline]
PHST- 2003/12/23 05:00 [entrez]
AID - S0165247803001962 [pii]
AID - 10.1016/j.imlet.2003.08.002 [doi]
PST - ppublish
SO  - Immunol Lett. 2003 Dec 15;90(2-3):215-21. doi: 10.1016/j.imlet.2003.08.002.