PMID- 14688336
OWN - NLM
STAT- MEDLINE
DCOM- 20040402
LR  - 20190516
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 172
IP  - 1
DP  - 2004 Jan 1
TI  - The alpha 1 beta 1 and alpha E beta 7 integrins define a subset of dendritic 
      cells in peripheral lymph nodes with unique adhesive and antigen uptake 
      properties.
PG  - 282-91
AB  - Dendritic cells (DCs) are a heterogeneous population of APCs with critical roles 
      in T cell activation and immune regulation. We report in this study the 
      identification and characterization of a novel subset of DCs resident in 
      skin-draining peripheral lymph nodes of normal mice. This subset of 
      CD11c(high)CD40(high)CD8alpha(intermediate (int)) DCs expresses the 
      collagen-binding integrin, alpha1beta1, and the E-cadherin-binding integrin, 
      alphaEbeta7. Although alpha1beta1 and alphaEbeta7 are also expressed on 
      CD11c(high)CD40(int)CD8alpha(high) lymphoid DCs, 
      CD11c(high)CD40(high)CD8alpha(int) DCs demonstrate preferential integrin-mediated 
      adhesion to collagen and fibronectin. This DC subset most likely acquires 
      expression of these integrins in peripheral lymph node, as this subset is not 
      found in the spleen or mesenteric lymph node, and recent DC migrants from the 
      skin lack expression of alpha1beta1 and alphaEbeta7 integrins. Resident 
      CD40(high) DCs express alpha1beta1 integrin and colocalize with collagen in lymph 
      nodes. When compared with CD11c(high)CD40(high)CD8alpha(int) DCs lacking 
      expression of these integrins, the alpha1beta1+alphaEbeta7+DC subset exhibits 
      more efficient formation of Ag-independent conjugates with T cells, and a 
      decreased ability to acquire soluble Ag. Thus, the alpha1beta1 and alphaEbeta7 
      integrins define a unique population of peripheral lymph node-derived DCs with 
      altered functional properties and adhesive potential that localizes these cells 
      to sites in lymph nodes where Ag presentation to T cells occurs.
FAU - Pribila, Jonathan T
AU  - Pribila JT
AD  - Department of Laboratory Medicine and Pathology, Center for Immunology, Cancer 
      Center, University of Minnesota Medical School, Minneapolis, MN 55455, USA.
FAU - Itano, Andrea A
AU  - Itano AA
FAU - Mueller, Kristen L
AU  - Mueller KL
FAU - Shimizu, Yoji
AU  - Shimizu Y
LA  - eng
GR  - AI 007313/AI/NIAID NIH HHS/United States
GR  - AI 31126/AI/NIAID NIH HHS/United States
GR  - AI 38474/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Antigens)
RN  - 0 (CD11c Antigen)
RN  - 0 (CD40 Antigens)
RN  - 0 (CD8 Antigens)
RN  - 0 (CD8alpha antigen)
RN  - 0 (Cadherins)
RN  - 0 (Epitopes, T-Lymphocyte)
RN  - 0 (Fibronectins)
RN  - 0 (Integrin alpha1beta1)
RN  - 0 (Integrin alpha2)
RN  - 0 (Integrins)
RN  - 0 (integrin alphaEbeta7)
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Animals
MH  - Antigens/*metabolism
MH  - CD11c Antigen/biosynthesis
MH  - CD40 Antigens/biosynthesis
MH  - CD8 Antigens/biosynthesis
MH  - Cadherins/metabolism
MH  - Cell Adhesion/immunology
MH  - Cell Movement/immunology
MH  - Collagen/metabolism
MH  - Dendritic Cells/*cytology/*immunology/metabolism
MH  - Epitopes, T-Lymphocyte/metabolism
MH  - Fibronectins/metabolism
MH  - Immunophenotyping
MH  - Integrin alpha1beta1/*biosynthesis/metabolism/physiology
MH  - Integrin alpha2/metabolism
MH  - Integrins/*biosynthesis/metabolism/physiology
MH  - Langerhans Cells/immunology/metabolism
MH  - Lymph Nodes/*cytology/*immunology/metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Transgenic
MH  - Protein Binding/immunology
MH  - Skin/cytology/immunology/metabolism
MH  - Solubility
EDAT- 2003/12/23 05:00
MHDA- 2004/04/03 05:00
CRDT- 2003/12/23 05:00
PHST- 2003/12/23 05:00 [pubmed]
PHST- 2004/04/03 05:00 [medline]
PHST- 2003/12/23 05:00 [entrez]
AID - 10.4049/jimmunol.172.1.282 [doi]
PST - ppublish
SO  - J Immunol. 2004 Jan 1;172(1):282-91. doi: 10.4049/jimmunol.172.1.282.