PMID- 14688370
OWN - NLM
STAT- MEDLINE
DCOM- 20040402
LR  - 20220804
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 172
IP  - 1
DP  - 2004 Jan 1
TI  - Soluble fractalkine prevents monocyte chemoattractant protein-1-induced monocyte 
      migration via inhibition of stress-activated protein kinase 2/p38 and matrix 
      metalloproteinase activities.
PG  - 585-92
AB  - In this study, we address the question of the cross-talk between two chemokines 
      that are cosecreted during inflammation, namely monocyte chemoattractant 
      protein-1 (MCP-1) and soluble fractalkine (s-FKN), toward monocyte migration. We 
      found that s-FKN fails to induce MonoMac6 cell migration per se. Interestingly, 
      this chemokine antagonizes transendothelial migration and chemotaxis of MonoMac6 
      cells and freshly isolated human monocytes induced by MCP-1, indicating a direct 
      effect of s-FKN on monocytic cells. In this study, we found that stress-activated 
      protein kinase (SAPK)1/c-Jun N-terminal kinase 1 and SAPK2/p38 are involved in 
      the control of MCP-1-induced MonoMac6 cell migration. We demonstrated that s-FKN 
      abrogates the MCP-1-induced SAPK2/p38 activation as well as the upstream Pyk2 
      activity. Furthermore, we observed that s-FKN also inhibits the activity of a 
      major matrix metalloproteinase (MMP), namely MMP-2. Taken collectively, our 
      results indicate that the s-FKN antagonizes the chemoattractant effect of MCP-1 
      on monocytes, likely by inhibiting crucial signaling pathways, like SAPK2/p38 and 
      MMP-2 activities.
FAU - Vitale, Sebastien
AU  - Vitale S
AD  - Institut National de la Sante et de la Recherche Medicale, Unite 364, Institut 
      Federatif de Recherche 50, Faculte de Medecine, Avenue de Valombrose, 06107 Nice 
      Cedex 02, France.
FAU - Schmid-Alliana, Annie
AU  - Schmid-Alliana A
FAU - Breuil, Veronique
AU  - Breuil V
FAU - Pomeranz, Manuel
AU  - Pomeranz M
FAU - Millet, Marie-Ange
AU  - Millet MA
FAU - Rossi, Bernard
AU  - Rossi B
FAU - Schmid-Antomarchi, Heidy
AU  - Schmid-Antomarchi H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (CX3CL1 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CX3CL1)
RN  - 0 (Chemokines, CX3C)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Matrix Metalloproteinase Inhibitors)
RN  - 0 (Membrane Proteins)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
RN  - EC 2.7.10.2 (Focal Adhesion Kinase 2)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
RN  - EC 3.4.24.24 (Matrix Metalloproteinase 2)
SB  - IM
MH  - Cell Line
MH  - Cell Line, Tumor
MH  - *Cell Migration Inhibition
MH  - Cell Separation
MH  - Chemokine CCL2/antagonists & inhibitors/*physiology
MH  - Chemokine CX3CL1
MH  - Chemokines, CX3C/*physiology
MH  - Chemotaxis, Leukocyte/*immunology
MH  - Endothelium, Vascular/cytology/immunology
MH  - Enzyme Activation/immunology
MH  - Enzyme Induction/immunology
MH  - Enzyme Inhibitors/pharmacology
MH  - Focal Adhesion Kinase 2
MH  - Humans
MH  - Matrix Metalloproteinase 2/biosynthesis/metabolism/physiology
MH  - *Matrix Metalloproteinase Inhibitors
MH  - Membrane Proteins/*physiology
MH  - Mitogen-Activated Protein Kinases/*antagonists & 
      inhibitors/biosynthesis/metabolism/physiology
MH  - Monocytes/cytology/*enzymology/immunology
MH  - Protein-Tyrosine Kinases/antagonists & inhibitors/biosynthesis/metabolism
MH  - Solubility
MH  - p38 Mitogen-Activated Protein Kinases
EDAT- 2003/12/23 05:00
MHDA- 2004/04/03 05:00
CRDT- 2003/12/23 05:00
PHST- 2003/12/23 05:00 [pubmed]
PHST- 2004/04/03 05:00 [medline]
PHST- 2003/12/23 05:00 [entrez]
AID - 10.4049/jimmunol.172.1.585 [doi]
PST - ppublish
SO  - J Immunol. 2004 Jan 1;172(1):585-92. doi: 10.4049/jimmunol.172.1.585.