PMID- 14688388
OWN - NLM
STAT- MEDLINE
DCOM- 20040812
LR  - 20211109
IS  - 1066-5099 (Print)
IS  - 1066-5099 (Linking)
VI  - 22
IP  - 1
DP  - 2004
TI  - Choroidal neovascularization is provided by bone marrow cells.
PG  - 21-6
AB  - Choroidal neovascularization (CNV) is a known cause of age-related macular 
      degeneration (ARMD). Moreover, the most common cause of blindness in the elderly 
      in advanced countries is ARMD with CNV. It has recently been shown that bone 
      marrow cells (BMCs) can differentiate into various cell lineages in vitro and in 
      vivo. Adults maintain a reservoir of hematopoietic stem cells included in BMCs 
      that can enter the circulation to reach various organs in need of regeneration. 
      It has recently been reported that endothelial progenitor cells (EPCs) included 
      in BMCs are associated with neovascularization. We examine the role of BMCs in 
      CNV using a model of CNV in adult mice. Using methods consisting of fractionated 
      irradiation (6.0 Gy x 2) followed by bone marrow transplantation (BMT), adult 
      mice were engrafted with whole BMCs isolated from transgenic mice expressing 
      enhanced green fluorescent protein (EGFP). Three months after BMT, we confirmed 
      that the hematopoietic cells in the recipients had been completely replaced with 
      donor cells. We then carried out laser photocoagulation to induce CNV in chimeric 
      mice (donor cells >95%). Two weeks after the laser photocoagulation, by which 
      time CNV had occurred, immunohistochemical examination was carried out. The 
      vascular wall cells of the CNV expressed both EGFP and CD31. These findings 
      indicate that newly developed blood vessels in the CNV are derived from the BMCs 
      and suggest that the inhibition of EPC mobilization from the bone marrow to the 
      eyes could be a new approach to the fundamental treatment of CNV in ARMD.
FAU - Tomita, Minoru
AU  - Tomita M
AD  - First Department of Pathology, Kansai Medical University, Moriguchi City, Osaka, 
      Japan.
FAU - Yamada, Haruhiko
AU  - Yamada H
FAU - Adachi, Yasushi
AU  - Adachi Y
FAU - Cui, Yunze
AU  - Cui Y
FAU - Yamada, Eri
AU  - Yamada E
FAU - Higuchi, Akiko
AU  - Higuchi A
FAU - Minamino, Keizo
AU  - Minamino K
FAU - Suzuki, Yasuhiko
AU  - Suzuki Y
FAU - Matsumura, Miyo
AU  - Matsumura M
FAU - Ikehara, Susumu
AU  - Ikehara S
LA  - eng
PT  - Journal Article
PL  - England
TA  - Stem Cells
JT  - Stem cells (Dayton, Ohio)
JID - 9304532
RN  - 0 (Luminescent Proteins)
RN  - 0 (Platelet Endothelial Cell Adhesion Molecule-1)
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
MH  - Animals
MH  - Blood Vessels/growth & development/metabolism/pathology
MH  - Cell Differentiation/*physiology
MH  - Cell Lineage/physiology
MH  - Cell Movement/drug effects/physiology
MH  - Choroidal Neovascularization/*etiology/pathology/*physiopathology
MH  - Disease Models, Animal
MH  - Endothelial Cells/pathology/*physiology
MH  - Graft Survival/physiology
MH  - Green Fluorescent Proteins
MH  - Hematopoietic Stem Cell Transplantation
MH  - Hematopoietic Stem Cells/cytology/pathology/*physiology
MH  - Light Coagulation/adverse effects
MH  - Luminescent Proteins/genetics/metabolism
MH  - Macular Degeneration/etiology
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Transgenic
MH  - Platelet Endothelial Cell Adhesion Molecule-1/metabolism
MH  - Radiation Chimera
EDAT- 2003/12/23 05:00
MHDA- 2004/08/13 05:00
CRDT- 2003/12/23 05:00
PHST- 2003/12/23 05:00 [pubmed]
PHST- 2004/08/13 05:00 [medline]
PHST- 2003/12/23 05:00 [entrez]
AID - 10.1634/stemcells.22-1-21 [doi]
PST - ppublish
SO  - Stem Cells. 2004;22(1):21-6. doi: 10.1634/stemcells.22-1-21.