PMID- 1469081
OWN - NLM
STAT- MEDLINE
DCOM- 19930122
LR  - 20190824
IS  - 0165-5728 (Print)
IS  - 0165-5728 (Linking)
VI  - 41
IP  - 2
DP  - 1992 Dec
TI  - Differential expression of heat shock proteins by human glial cells.
PG  - 231-8
AB  - Heat shock proteins (HSP) have been implicated in the interactions between the 
      gamma delta T lymphocyte population and target tissues. gamma delta T cells are 
      found in increased numbers in multiple sclerosis (MS) plaques compared to their 
      proportion in peripheral blood, co-localizing with oligodendrocytes (OGC) 
      expressing HSP. We have demonstrated that such gamma delta T cells can induce in 
      vitro lysis of human adult-derived OGC. Using immunohistochemical and flow 
      cytometry techniques, we examined the constitutive and/or inducible expression of 
      HSP in or on adult human-derived glial cell cultures in vitro. HSP70 was 
      expressed in OGC maintained at basal temperature, but the expression of the 
      inducible HSP70 protein was upregulated by a prior 43 degrees C heat exposure. 
      HSP70 could not be detected within astrocytes (GFAP+ cells), whether heat stress 
      was applied or not. Constitutive expression of HSP60 could be discerned on the 
      surface of all OGC under non-stressed culture conditions. Only some astrocytes 
      demonstrated minor punctate surface HSP60 staining, whereas the remainder did not 
      express HSP60 constitutively. These observations raise the possibility that OGC, 
      by virtue of their differential expression of HSP compared to other glial cells, 
      may be particularly prone to interaction with HSP-reactive gamma delta T cells. 
      Such findings may further implicate gamma delta T cells in the pathogenesis of 
      MS, a putative autoimmune disease in which immune-mediated injury is directed 
      specifically against the oligodendrocyte-myelin unit within the central nervous 
      system.
FAU - Freedman, M S
AU  - Freedman MS
AD  - Department of Neurology and Neurosurgery, McGill University, Montreal 
      Neurological Institute, Quebec, Canada.
FAU - Buu, N N
AU  - Buu NN
FAU - Ruijs, T C
AU  - Ruijs TC
FAU - Williams, K
AU  - Williams K
FAU - Antel, J P
AU  - Antel JP
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - J Neuroimmunol
JT  - Journal of neuroimmunology
JID - 8109498
RN  - 0 (Heat-Shock Proteins)
RN  - 0 (Receptors, Antigen, T-Cell, gamma-delta)
SB  - IM
MH  - Adult
MH  - Cells, Cultured
MH  - Heat-Shock Proteins/*biosynthesis
MH  - Humans
MH  - Multiple Sclerosis/etiology/immunology
MH  - Neuroglia/*metabolism
MH  - Oligodendroglia/metabolism
MH  - Receptors, Antigen, T-Cell, gamma-delta/physiology
MH  - T-Lymphocytes/physiology
EDAT- 1992/12/01 00:00
MHDA- 2000/06/01 00:00
CRDT- 1992/12/01 00:00
PHST- 1992/12/01 00:00 [pubmed]
PHST- 2000/06/01 00:00 [medline]
PHST- 1992/12/01 00:00 [entrez]
AID - 0165-5728(92)90074-U [pii]
AID - 10.1016/0165-5728(92)90074-u [doi]
PST - ppublish
SO  - J Neuroimmunol. 1992 Dec;41(2):231-8. doi: 10.1016/0165-5728(92)90074-u.