PMID- 1469085
OWN - NLM
STAT- MEDLINE
DCOM- 19930127
LR  - 20181113
IS  - 0021-9738 (Print)
IS  - 0021-9738 (Linking)
VI  - 90
IP  - 6
DP  - 1992 Dec
TI  - Entactin stimulates neutrophil adhesion and chemotaxis through interactions 
      between its Arg-Gly-Asp (RGD) domain and the leukocyte response integrin.
PG  - 2251-7
AB  - Entactin is an integral component of basement membranes that plays a major role 
      in basement membrane assembly through its ability to bind avidly to both laminin 
      and type IV collagen. Because neutrophil (PMN) interactions with entactin have 
      not been examined, we investigated the ability of natural and recombinant 
      entactin to mediate PMN adhesion and chemotaxis. With both forms of entactin, we 
      observed that entactin-coated surfaces promoted PMN adhesion and that entactin 
      stimulated PMN chemotaxis. The increase in adhesion to entactin over control was 
      two to threefold whereas the chemotactic response to 15 ng/ml (1 x 10(-10) M) 
      entactin was equivalent to the chemotactic response elicited with 1 x 10(-8) M 
      formyl-methionyl-leucyl-phenylalanine (fMLP). HL-60 cells, after differentiation 
      with dimethylsulfoxide, also demonstrated adhesion and chemotaxis to entactin. A 
      synthetic peptide of the Arg-Gly-Asp (RGD) domain in entactin, SIGFRGDGQTC 
      (S-RGD), mediated PMN adhesion and chemotaxis, and preexposure of PMN to S-RGD 
      blocked PMN adhesion and chemotaxis induced by entactin without diminishing the 
      adhesive and chemotactic activities of fMLP. In contrast, preexposure to peptides 
      SIGFRGEGQTCA or SIGFKGDGQTCA had no effect. The findings with synthetic peptides 
      were confirmed with a recombinant entactin mutant in which aspartic acid at 
      residue 674 was replaced with glutamic acid, thus converting the RGD sequence of 
      entactin to RGE. RGE-entactin was neither adhesive nor chemotactic for 
      neutrophils. Monoclonal antibodies to the leukocyte response integrin (LRI) and 
      the integrin-associated protein blocked entactin-mediated adhesion and chemotaxis 
      whereas monoclonal antibodies to beta 1 and beta 2 integrins had no effect and 
      PMN from an individual with leukocyte-adhesion deficiency adhered normally to 
      entactin-coated surfaces. These data demonstrate that entactin mediates 
      biologically and pathologically important functions of PMN through its RGD domain 
      and that LRI, which has been shown previously to mediate RGD-stimulated 
      phagocytosis, is also capable of mediating RGD-stimulated PMN adhesion and 
      chemotaxis.
FAU - Senior, R M
AU  - Senior RM
AD  - Department of Medicine, Jewish Hospital, Washington University Medical Center, 
      St. Louis, Missouri 63110.
FAU - Gresham, H D
AU  - Gresham HD
FAU - Griffin, G L
AU  - Griffin GL
FAU - Brown, E J
AU  - Brown EJ
FAU - Chung, A E
AU  - Chung AE
LA  - eng
GR  - AI 23790/AI/NIAID NIH HHS/United States
GR  - GM 38330/GM/NIGMS NIH HHS/United States
GR  - HL 29594/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Clin Invest
JT  - The Journal of clinical investigation
JID - 7802877
RN  - 0 (Integrins)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (Oligopeptides)
RN  - 0 (nidogen)
RN  - 78VO7F77PN (arginyl-glycyl-aspartic acid)
RN  - 9001-32-5 (Fibrinogen)
SB  - IM
MH  - Amino Acid Sequence
MH  - Cell Adhesion/*drug effects
MH  - Chemotaxis, Leukocyte/*drug effects
MH  - Fibrinogen/physiology
MH  - Humans
MH  - Immunologic Techniques
MH  - In Vitro Techniques
MH  - Integrins/*physiology
MH  - Membrane Glycoproteins/*pharmacology
MH  - Membrane Proteins/metabolism
MH  - Molecular Sequence Data
MH  - Neutrophils/*physiology
MH  - Oligopeptides
MH  - Structure-Activity Relationship
MH  - Tumor Cells, Cultured
PMC - PMC443376
EDAT- 1992/12/01 00:00
MHDA- 1992/12/01 00:01
PMCR- 1992/12/01
CRDT- 1992/12/01 00:00
PHST- 1992/12/01 00:00 [pubmed]
PHST- 1992/12/01 00:01 [medline]
PHST- 1992/12/01 00:00 [entrez]
PHST- 1992/12/01 00:00 [pmc-release]
AID - 10.1172/JCI116111 [doi]
PST - ppublish
SO  - J Clin Invest. 1992 Dec;90(6):2251-7. doi: 10.1172/JCI116111.