PMID- 14693736
OWN - NLM
STAT- MEDLINE
DCOM- 20040423
LR  - 20151119
IS  - 1055-9965 (Print)
IS  - 1055-9965 (Linking)
VI  - 12
IP  - 12
DP  - 2003 Dec
TI  - Immune profiling of plasma and cervical secretions using recycling immunoaffinity 
      chromatography.
PG  - 1449-56
AB  - Small volumes of cervical secretions have limited measurements of immunity at the 
      cervix, which may be important to studies of human papillomavirus (HPV). We 
      report the use of recycling immunoaffinity chromatography to efficiently study 
      immune profiles in cervical secretions. Frozen pairs of plasma and cervical 
      secretions (collected on ophthalmic sponges) were selected randomly from women 
      with normal cervical cytology (n = 50) participating in a natural history study 
      of HPV in Guanacaste, Costa Rica. Single 25- micro l aliquots of plasma and 
      (diluted) cervical secretions were assayed for interleukin (IL) -1 beta, -2, -4, 
      -6, -8, -10, -12, -13, -15, IFN-alpha, -beta, -gamma, tumor necrosis 
      factor-alpha, -beta, RANTES (regulated on activation normal T-cell express and 
      secreted), MCP-1 (monocyte chemoattractant protein), -2, -3, macrophage 
      inflammatory protein-1 alpha, -1 beta (regulated on activation normal T-cell 
      express and secreted), macrophage colony-stimulating factor, IgG, IgA, and 
      cyclooxygenase 2. All of the specimens were tested as blind replicates, and 
      refrozen plasma was retested 4 months later. To evaluate the reproducibility of 
      the repeat measurements and to examine the correlation between plasma and 
      cervical secretions, we calculated kappa values with 95% confidence intervals 
      among categorized analyte values and Spearman correlation coefficients (rho) 
      among detectable, continuous analyte values. Measurements of all of the analytes 
      in either plasma or cervical secretions were highly reproducible, with all of the 
      kappa > or = 0.78 (70% above 0.90), and all of the rho > or = 0.88 (96% above 
      0.90). Only IL-1 beta (kappa = 0.60 and rho = 0.82) and IL-6 (kappa = 0.50 and 
      rho = 0.78) levels were strongly correlated between plasma and cervical 
      secretions. IFN-gamma, tumor necrosis factor-beta, RANTES, MCP-1, MCP -2, 
      macrophage inflammatory protein-1 alpha, and macrophage colony-stimulating factor 
      levels were especially poorly correlated between plasma and cervical secretions 
      (kappa < or = 0.25 and rho < or = 0.25). We conclude that recycling 
      immunoaffinity chromatography is a reproducible method of measuring immune 
      profiles from biological specimens, and immune profiles are not well correlated 
      between plasma and cervical secretions, perhaps necessitating cervical 
      collections to study cervix-specific immunity in HPV natural history studies.
FAU - Castle, Philip E
AU  - Castle PE
AD  - Division of Cancer Epidemiology and Genetics, National Cancer Institute, National 
      Institutes of Health, Department of Health and Human Services, Bethesda, Maryland 
      20892-7234, USA. castlep@mail.nih.gov
FAU - Phillips, Terry M
AU  - Phillips TM
FAU - Hildesheim, Allan
AU  - Hildesheim A
FAU - Herrero, Rolando
AU  - Herrero R
FAU - Bratti, M Concepcion
AU  - Bratti MC
FAU - Rodriguez, Ana Cecilia
AU  - Rodriguez AC
FAU - Morera, Lidia Ana
AU  - Morera LA
FAU - Pfeiffer, Ruth
AU  - Pfeiffer R
FAU - Hutchinson, Martha L
AU  - Hutchinson ML
FAU - Pinto, Ligia A
AU  - Pinto LA
FAU - Schiffman, Mark
AU  - Schiffman M
LA  - eng
GR  - N01CP2101/CP/NCI NIH HHS/United States
GR  - N01CP31061/CP/NCI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cancer Epidemiol Biomarkers Prev
JT  - Cancer epidemiology, biomarkers & prevention : a publication of the American 
      Association for Cancer Research, cosponsored by the American Society of 
      Preventive Oncology
JID - 9200608
RN  - 0 (Biomarkers)
RN  - 0 (CCL8 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CCL5)
RN  - 0 (Chemokine CCL8)
RN  - 0 (Monocyte Chemoattractant Proteins)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Adult
MH  - Age Distribution
MH  - Aged
MH  - Biomarkers/*blood
MH  - Cervix Mucus/virology
MH  - Chemokine CCL2/analysis
MH  - Chemokine CCL5/analysis
MH  - Chemokine CCL8
MH  - Chromatography/*methods
MH  - Cohort Studies
MH  - Confidence Intervals
MH  - Costa Rica/epidemiology
MH  - Female
MH  - Humans
MH  - Immunologic Techniques
MH  - Incidence
MH  - Mass Screening/methods
MH  - Middle Aged
MH  - Monocyte Chemoattractant Proteins/analysis
MH  - Papillomaviridae/*isolation & purification
MH  - Papillomavirus Infections/*diagnosis/*epidemiology
MH  - Probability
MH  - Risk Factors
MH  - Specimen Handling
MH  - Tumor Necrosis Factor-alpha/analysis
EDAT- 2003/12/25 05:00
MHDA- 2004/04/24 05:00
CRDT- 2003/12/25 05:00
PHST- 2003/12/25 05:00 [pubmed]
PHST- 2004/04/24 05:00 [medline]
PHST- 2003/12/25 05:00 [entrez]
PST - ppublish
SO  - Cancer Epidemiol Biomarkers Prev. 2003 Dec;12(12):1449-56.