PMID- 14694363
OWN - NLM
STAT- MEDLINE
DCOM- 20040226
LR  - 20190710
IS  - 1531-5037 (Electronic)
IS  - 0022-3468 (Linking)
VI  - 39
IP  - 1
DP  - 2004 Jan
TI  - Glucocorticoid treatment down-regulates chemokine expression of bacterial 
      cholangitis in cholestatic rats.
PG  - 10-5
AB  - BACKGROUND: Postoperative cholangitis is common after operation for biliary 
      atresia. Empirical pulse therapy with glucocorticoid is effective in reversing 
      some detrimental clinical manifestations, but the rationale for such a therapy 
      still is not substantiated. METHODS: Adult male rats were divided into groups 
      according to the treatment: sterile normal saline (NS) or Escherichia coli (EC, 1 
      mL containing 10(8) cells of ATCC 25922 strain), 1 mL, were infused into the 
      proximal choledochostomy (PC) tube 2 weeks after ligation of the PC tube (bile 
      duct ligation, BDL), then immediate tube-tube choledocho-choledochostomy (biliary 
      drainage, BD) was constructed. A high dose of dexamethasone (DEX, intraperitoneal 
      injection; 2 mg/kg of body weight) was given after BD in treatment groups. 
      Histopathology of the liver, as well as liver chemokine mRNA expression and serum 
      chemokine levels, were studied 24 hours after treatment. RESULTS: Inflammatory 
      cell infiltration to the liver was retarded with DEX treatment, which was 
      correlated with a significantly lower expression of interleukin-8 (IL-8) and 
      monocyte chemoattractant protein-1 (MCP-1) mRNA in the liver (P =.006). Serum 
      IL-8 and MCP-1 levels were also significantly down-regulated with DEX treatment 
      (P = 0.008). CONCLUSIONS: Glucocorticoid treatment is effective in modulating 
      IL-8 and MCP-1 expression and ameliorating inflammatory cell infiltration in rat 
      liver with bacterial cholangitis and cholestasis.
FAU - Hsieh, Chih-Sung
AU  - Hsieh CS
AD  - Department of Pediatric Surgery, Chang Gung Memorial Hospital & Change Gung 
      University, Naio-Sung Hsiang, Kaohsiung Hsien, Taiwan.
FAU - Huang, Chao-Cheng
AU  - Huang CC
FAU - Huang, Li-Tung
AU  - Huang LT
FAU - Tsai, Yi-Ju
AU  - Tsai YJ
FAU - Chou, Ming-Huei
AU  - Chou MH
FAU - Chuang, Jiin-Haur
AU  - Chuang JH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Pediatr Surg
JT  - Journal of pediatric surgery
JID - 0052631
RN  - 0 (Chemokine CCL2)
RN  - 0 (Glucocorticoids)
RN  - 0 (Interleukin-8)
RN  - 7S5I7G3JQL (Dexamethasone)
SB  - IM
MH  - Animals
MH  - Bacterial Infections/drug therapy/*metabolism
MH  - Biliary Atresia/drug therapy/surgery
MH  - Chemokine CCL2/*metabolism
MH  - Cholangitis/drug therapy/etiology/*metabolism
MH  - Cholestasis/complications/drug therapy/metabolism
MH  - Dexamethasone/*pharmacology/therapeutic use
MH  - Disease Models, Animal
MH  - Down-Regulation
MH  - Glucocorticoids/*pharmacology/therapeutic use
MH  - Interleukin-8/*metabolism
MH  - Liver/metabolism
MH  - Male
MH  - Postoperative Complications/drug therapy/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 2003/12/25 05:00
MHDA- 2004/02/27 05:00
CRDT- 2003/12/25 05:00
PHST- 2003/12/25 05:00 [pubmed]
PHST- 2004/02/27 05:00 [medline]
PHST- 2003/12/25 05:00 [entrez]
AID - S0022346803007048 [pii]
AID - 10.1016/j.jpedsurg.2003.09.012 [doi]
PST - ppublish
SO  - J Pediatr Surg. 2004 Jan;39(1):10-5. doi: 10.1016/j.jpedsurg.2003.09.012.