PMID- 14694501
OWN - NLM
STAT- MEDLINE
DCOM- 20040206
LR  - 20220408
IS  - 0148-639X (Print)
IS  - 0148-639X (Linking)
VI  - 29
IP  - 1
DP  - 2004 Jan
TI  - Exercise-induced gene expression in soleus muscle is dependent on time after 
      spinal cord injury in rats.
PG  - 73-81
AB  - Cycling exercise attenuates atrophy in hindlimb muscles and causes changes in 
      spinal cord properties after spinal cord injury in rats. We hypothesized that 
      exercising soleus muscle expresses genes that are potentially beneficial to the 
      injured spinal cord. Rats underwent spinal cord injury at T10 and were exercised 
      on a motor-driven bicycle. Soleus muscle and lumbar spinal cord tissue were used 
      for messenger RNA (mRNA) analysis. Gene expression of brain-derived neurotrophic 
      factor (BDNF) and glial cell line-derived neurotrophic factor (GDNF) was elevated 
      11- and 14-fold, respectively, in soleus muscle after one bout of exercise 
      performed 5 days after spinal cord transection. Also, c-fos and heat shock 
      protein-27 (HSP27) mRNA abundance were increased 11- and 7-fold, respectively. 
      When exercise was started 2 days after the injury, the changes in gene expression 
      were not observed. By contrast, at 2 but not at 5 days after transection, 
      expression of the HSP27 gene was elevated sixfold in the lumbar spinal cord, 
      independent of exercise. Electromyographic activity in soleus muscles was also 
      decreased at 2 days, indicating that the spinal cord was less permissive to 
      exercise at this early time. Long-term exercise for 4 weeks attenuated muscle 
      atrophy equally well in rats started at 2 days or 5 days after injury. We 
      conclude that BDNF and GDNF released from exercising muscle may be involved in 
      exercise-induced plasticity of the spinal cord. Furthermore, the data suggest 
      that the lumbar spinal cord undergoes time-dependent changes that temporarily 
      impede the ability of the muscle to respond to exercise.
FAU - Dupont-Versteegden, Esther E
AU  - Dupont-Versteegden EE
AD  - Department of Geriatrics, University of Arkansas for Medical Sciences, 4301 West 
      Markham, Little Rock, Arkansas 72205, USA. duponttesthere@uams.edu
FAU - Houle, John D
AU  - Houle JD
FAU - Dennis, Richard A
AU  - Dennis RA
FAU - Zhang, Junming
AU  - Zhang J
FAU - Knox, Micheal
AU  - Knox M
FAU - Wagoner, Gail
AU  - Wagoner G
FAU - Peterson, Charlotte A
AU  - Peterson CA
LA  - eng
GR  - NS40008/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Muscle Nerve
JT  - Muscle & nerve
JID - 7803146
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Gdnf protein, rat)
RN  - 0 (Glial Cell Line-Derived Neurotrophic Factor)
RN  - 0 (HSP27 Heat-Shock Proteins)
RN  - 0 (Heat-Shock Proteins)
RN  - 0 (Hspb1 protein, rat)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Proto-Oncogene Proteins c-fos)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Action Potentials/physiology
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/genetics
MH  - Female
MH  - Glial Cell Line-Derived Neurotrophic Factor
MH  - HSP27 Heat-Shock Proteins
MH  - *Heat-Shock Proteins
MH  - Muscle, Skeletal/innervation/*metabolism/physiopathology
MH  - Muscular Atrophy/*etiology/*metabolism/therapy
MH  - Neoplasm Proteins/genetics
MH  - Nerve Growth Factors/*genetics
MH  - Neuronal Plasticity/genetics
MH  - *Physical Conditioning, Animal
MH  - Proto-Oncogene Proteins c-fos/genetics
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reaction Time/genetics
MH  - Spinal Cord/metabolism/physiopathology
MH  - Spinal Cord Injuries/*complications
MH  - Time Factors
MH  - Up-Regulation/genetics
EDAT- 2003/12/25 05:00
MHDA- 2004/02/10 05:00
CRDT- 2003/12/25 05:00
PHST- 2003/12/25 05:00 [pubmed]
PHST- 2004/02/10 05:00 [medline]
PHST- 2003/12/25 05:00 [entrez]
AID - 10.1002/mus.10511 [doi]
PST - ppublish
SO  - Muscle Nerve. 2004 Jan;29(1):73-81. doi: 10.1002/mus.10511.