PMID- 14694519
OWN - NLM
STAT- MEDLINE
DCOM- 20040209
LR  - 20061115
IS  - 0022-3417 (Print)
IS  - 0022-3417 (Linking)
VI  - 202
IP  - 1
DP  - 2004 Jan
TI  - Expression of interleukin-6 and monocyte chemoattractant protein-1 by peritoneal 
      sub-mesothelial cells during abdominal operations.
PG  - 34-40
AB  - Cytokines and chemokines including interleukin-6 (IL-6) and monocyte 
      chemoattractant protein-1 (MCP-1) are secreted in response to major abdominal 
      operations. The aim of this study was to identify the peritoneal cells that 
      produce IL-6 and MCP-1. Samples of peritoneal tissue were taken from patients at 
      the beginning and end of major abdominal operations. The samples were incubated 
      in culture medium on microtitre plates for 5 h. The concentrations of IL-6 and 
      MCP-1 were measured in culture supernatants by enzyme-linked immunosorbent assay 
      (ELISA). In paraffin sections, cells that expressed IL-6 or MCP-1 were identified 
      by combined in situ hybridization and immunohistochemistry. Antibodies against 
      CD68, CD34, actin, and calretinin were included in these experiments. The median 
      production of IL-6 increased significantly from 6256 pg/ml at the start of the 
      operation to 20,000 pg/ml at the end. Production of MCP-1 rose from 7700 pg/ml to 
      11,820 pg/ml. IL-6 mRNA was mainly confined to endothelial cells. MCP-1 was 
      expressed by a broader range of cells, consisting of actin-positive smooth muscle 
      cells and endothelial cells, fibroblast-like cells, as well as occasional 
      macrophages and mesothelial cells. Peritoneal endothelial cells contribute to the 
      transient increase in concentrations of IL-6 in the circulation after surgical 
      trauma. Recruitment of monocytes to the site of the trauma seems to be mainly 
      effected by actin-positive smooth muscle cells and endothelial cells.
CI  - Copyright 2003 John Wiley & Sons, Ltd.
FAU - Riese, J
AU  - Riese J
AD  - University of Erlangen-Nuremberg, Department of Surgery, Germany. 
      jutta.riese@charite.de
FAU - Niedobitek, G
AU  - Niedobitek G
FAU - Lisner, R
AU  - Lisner R
FAU - Jung, A
AU  - Jung A
FAU - Hohenberger, W
AU  - Hohenberger W
FAU - Haupt, W
AU  - Haupt W
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Pathol
JT  - The Journal of pathology
JID - 0204634
RN  - 0 (Chemokine CCL2)
RN  - 0 (Interleukin-6)
RN  - 63231-63-0 (RNA)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Cells, Cultured
MH  - Chemokine CCL2/*analysis
MH  - Endothelial Cells/metabolism
MH  - Enzyme-Linked Immunosorbent Assay/methods
MH  - Epithelial Cells/metabolism
MH  - Female
MH  - Fibroblasts/metabolism
MH  - Gene Expression/genetics
MH  - Humans
MH  - Immunohistochemistry/methods
MH  - In Situ Hybridization/methods
MH  - Interleukin-6/*analysis
MH  - Macrophages/metabolism
MH  - Male
MH  - Middle Aged
MH  - Muscle, Smooth/metabolism
MH  - Pancreatic Neoplasms/pathology/surgery
MH  - Peritoneum/*metabolism/pathology/surgery
MH  - Phenotype
MH  - RNA/analysis
MH  - Stomach Neoplasms/pathology/surgery
EDAT- 2003/12/25 05:00
MHDA- 2004/02/11 05:00
CRDT- 2003/12/25 05:00
PHST- 2003/12/25 05:00 [pubmed]
PHST- 2004/02/11 05:00 [medline]
PHST- 2003/12/25 05:00 [entrez]
AID - 10.1002/path.1455 [doi]
PST - ppublish
SO  - J Pathol. 2004 Jan;202(1):34-40. doi: 10.1002/path.1455.