PMID- 14695763
OWN - NLM
STAT- MEDLINE
DCOM- 20040211
LR  - 20190430
IS  - 1007-9327 (Print)
IS  - 2219-2840 (Electronic)
IS  - 1007-9327 (Linking)
VI  - 10
IP  - 1
DP  - 2004 Jan
TI  - Construction and identification of recombinant vectors carrying herpes simplex 
      virus thymidine kinase and cytokine genes expressed in gastric carcinoma cell 
      line SGC7901.
PG  - 26-30
AB  - AIM: To construct and identify the recombinant vectors carrying herpes simplex 
      virus thymidine kinase (HSV-TK) and tumor necrosis factor alpha (TNF-alpha) or 
      interleukin-2 (IL-2) genes expressed in gastric carcinoma cell line SGC7901. 
      METHODS: The fragments of HSV-TK, internal ribosome entry sites (IRES) and 
      TNF-alpha or IL-2 genes were inserted in a TK-IRES-TNF-alpha or TK-IRES-IL-2 
      order into pEGFP-N(3) and pLXSN to generate the therapeutic vectors pEGFP-TT, 
      pEGFP-TI, pL(TT)SN and pL(TI)SN respectively, which were structurally confirmed 
      by the digestion analysis of restriction endonuclease. The former two plasmids 
      were used for the transient expression of recombinant proteins in the target 
      cells while pL(TT)SN and pL(TI)SN were transfected into SGC7901 cells by 
      lipofectamine for the stable expression of objective genes through G418 
      selection. The protein products expressed transiently and stably in SGC7901 cells 
      by the constructed vectors were confirmed by fluorescent microscopy and Western 
      blot respectively. RESULTS: The inserted fragments in all constructed plasmids 
      were structurally confirmed to be consistent with that of the published data. In 
      the transient expression, both pEGFP-TT and pEGFP-TI were shown expressed in 
      nearly 50% of the transfected SGC7901 cells. Similarly, the G418 selected vectors 
      PL(TT)SN and PL(TI)SN were confirmed to be successful in the stable expression of 
      the objective proteins in the target cells. CONCLUSION: The constructed 
      recombinant vectors in the present study that can express the suicide gene TK in 
      combination with cytokines genes may serve as the potential tools to perform more 
      effective investigations in future for the gene therapy of gastric carcinoma.
FAU - Zhang, Jian-Hua
AU  - Zhang JH
AD  - Department of Biomedical Engineering, School of Life Science and Technology, 
      Xi'an Jiaotong University, 28 West Xianning Road, Xi'an 710049, Shaanxi Province, 
      China.
FAU - Wan, Ming-Xi
AU  - Wan MX
FAU - Yuan, Jia-Ying
AU  - Yuan JY
FAU - Pan, Bo-Rong
AU  - Pan BR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - World J Gastroenterol
JT  - World journal of gastroenterology
JID - 100883448
RN  - 0 (DNA, Recombinant)
RN  - 0 (Indicators and Reagents)
RN  - 0 (Interleukin-2)
RN  - 0 (Luminescent Proteins)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - EC 2.7.1.21 (Thymidine Kinase)
SB  - IM
MH  - Cell Line, Tumor
MH  - Cloning, Molecular/methods
MH  - DNA, Recombinant
MH  - Gene Expression Regulation, Viral
MH  - *Genetic Vectors
MH  - Green Fluorescent Proteins
MH  - Humans
MH  - Indicators and Reagents/metabolism
MH  - Interleukin-2/*genetics
MH  - Luminescent Proteins/genetics
MH  - Simplexvirus/*genetics
MH  - *Stomach Neoplasms
MH  - Thymidine Kinase/*genetics
MH  - Tumor Necrosis Factor-alpha/*genetics
PMC - PMC4717072
EDAT- 2003/12/27 05:00
MHDA- 2004/02/12 05:00
PMCR- 2004/01/01
CRDT- 2003/12/27 05:00
PHST- 2003/12/27 05:00 [pubmed]
PHST- 2004/02/12 05:00 [medline]
PHST- 2003/12/27 05:00 [entrez]
PHST- 2004/01/01 00:00 [pmc-release]
AID - 10.3748/wjg.v10.i1.26 [doi]
PST - ppublish
SO  - World J Gastroenterol. 2004 Jan;10(1):26-30. doi: 10.3748/wjg.v10.i1.26.