PMID- 14697268
OWN - NLM
STAT- MEDLINE
DCOM- 20040305
LR  - 20190901
IS  - 0031-9422 (Print)
IS  - 0031-9422 (Linking)
VI  - 65
IP  - 1
DP  - 2004 Jan
TI  - Purification and characterization of phytocystatins from kiwifruit cortex and 
      seeds.
PG  - 19-30
AB  - Kiwifruit cysteine proteinase inhibitors (KCPIs) were purified from the cortex 
      and seeds of kiwifruit after inactivation of the abundant cortex cysteine 
      proteinase actinidain. One major (KCPI1) and four minor cystatins were identified 
      from Actinidia deliciosa ripe mature kiwifruit cortex as well as a seed KCPI from 
      A. chinensis. The predominant cortex cystatin, KCPI1, inhibited clan CA, family 
      C1 (papain family) cysteine proteinases (papain, chymopapain, bromelain, ficin, 
      human cathepsins B, H and L, actinidain and the house dust mite endopeptidase 1), 
      while cysteine proteinases belonging to other families, [clostripain (C11), 
      streptopain (C10) and calpain (C2)] were not inhibited. Inhibition constants 
      (K(I)) ranged between 0.001 nM for cathepsin L and 0.98 nM for endopeptidase 1. 
      The K(I) (14 nM) for KCPI1 inhibiting actinidain is at least 2 orders of 
      magnitude higher than for other plant proteinases measured. The cortex KCPI1 and 
      a seed KCPI purified from seeds had the same N-terminal sequence 
      (VAAGGWRPIESLNSAEVQDV). BLAST-matching the peptide sequence against an in-house 
      generated Actinidia EST database, identified 81 cDNAs that exactly matched the 
      measured KCPI1 peptide sequence. Peptide sequences of two other cortex KCPIs each 
      exactly matched a predicted peptide sequence of a cDNA from kiwifruit. The 
      predicted peptide sequence of KCPI1 of 116 amino acids encodes a signal peptide 
      and does not contain cysteine. Without the signal peptide (mature protein), KCPI1 
      has a molecular mass of approximately 11 kDa, possesses the consensus sequence 
      characteristic for the phytocystatins and shows the highest homology to a 
      cystatin from Citrusxparadisi (52% identity). This is the first report of 
      phytocystatins from the Ericales.
FAU - Rassam, Maysoon
AU  - Rassam M
AD  - Gene Technologies, The Horticultural and Food Research Institute of New Zealand, 
      PB 92169, Auckland, New Zealand. mrassam@hortresearch.co.nz
FAU - Laing, William A
AU  - Laing WA
LA  - eng
SI  - GENBANK/AY390352
SI  - GENBANK/AY390353
SI  - GENBANK/AY390354
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Phytochemistry
JT  - Phytochemistry
JID - 0151434
RN  - 0 (Cystatins)
RN  - 0 (Cysteine Proteinase Inhibitors)
RN  - EC 3.4.22.- (Cysteine Endopeptidases)
SB  - IM
MH  - Actinidia/*chemistry
MH  - Amino Acid Sequence
MH  - Chromatography, High Pressure Liquid/methods
MH  - Cluster Analysis
MH  - Cystatins/genetics/*isolation & purification/*pharmacology
MH  - Cysteine Endopeptidases/metabolism
MH  - Cysteine Proteinase Inhibitors/isolation & purification/pharmacology
MH  - Fruit/*chemistry
MH  - Humans
MH  - Molecular Sequence Data
MH  - Seeds/chemistry
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods
EDAT- 2003/12/31 05:00
MHDA- 2004/03/06 05:00
CRDT- 2003/12/31 05:00
PHST- 2003/12/31 05:00 [pubmed]
PHST- 2004/03/06 05:00 [medline]
PHST- 2003/12/31 05:00 [entrez]
AID - S0031942203005983 [pii]
AID - 10.1016/j.phytochem.2003.09.019 [doi]
PST - ppublish
SO  - Phytochemistry. 2004 Jan;65(1):19-30. doi: 10.1016/j.phytochem.2003.09.019.