PMID- 14697637
OWN - NLM
STAT- MEDLINE
DCOM- 20040304
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 148
IP  - 1
DP  - 2004 Jan 1
TI  - Cytogenetic and fluorescence in situ hybridization characterization of clonal 
      chromosomal aberrations and CCND1 amplification in esophageal carcinomas.
PG  - 21-8
AB  - Cytogenetic analyses of four squamous cell carcinomas (SCC) of the esophagus 
      showed complex numerical and structural abnormalities. Chromosomal bands or 
      regions preferentially involved were 11q13, 8q10, 21q10, 3p10 approximately p11, 
      1p11 approximately q11, 5p11 approximately q11, and 14p11 approximately q11. For 
      the first time, to our knowledge, recurrent aberrations were identified in 
      esophageal SCC, including homogeneous staining region (hsr), isochromosomes i(3q) 
      and i(21q), and ring chromosome. Losses of chromosomal material dominated over 
      gains. Recurrent imbalances included under-representation of 4p13 approximately 
      pter, 5q14 approximately qter, 9p22 approximately pter, 10p, 11p13 approximately 
      pter, 12p13 approximately pter, 17p10 approximately pter, 18p11 approximately 
      pter, 21p, and 22p, as well as over-representation of 1q25 approximately qter, 
      3q, 7q, and 8q. Interestingly, hsr at different chromosomal regions occurred in 
      three of four cases. With the application of fluorescence in situ hybridization 
      (FISH) and multicolor combined binary ratio labeling-FISH with specific DNA 
      probes, it could be shown that in two cases the hsr was derived from chromosome 
      11 material and that the amplicon included CCND1. Our results, together with 
      previous molecular genetic findings, indicate that CCND1might be a prime target 
      in 11q13 amplification, and that amplification of this gene might be crucial in 
      the tumorigenesis of esophageal SCC. These observed chromosomal aberrations and 
      imbalances thus provide important information for further molecular genetic 
      investigation of esophageal SCC.
FAU - Jin, Yuesheng
AU  - Jin Y
AD  - Department of Medicine, Queen Mary Hospital, Pokfulam Road, Hong Kong, China.
FAU - Jin, Charlotte
AU  - Jin C
FAU - Law, Simon
AU  - Law S
FAU - Chu, Kent-Man
AU  - Chu KM
FAU - Zhang, Hao
AU  - Zhang H
FAU - Strombeck, Bodil
AU  - Strombeck B
FAU - Yuen, Anthony P W
AU  - Yuen AP
FAU - Kwong, Yok-Lam
AU  - Kwong YL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 136601-57-5 (Cyclin D1)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Carcinoma, Squamous Cell/*genetics
MH  - *Chromosome Aberrations
MH  - Cyclin D1/*genetics
MH  - Esophageal Neoplasms/*genetics
MH  - Female
MH  - *Gene Amplification
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Karyotyping
MH  - Male
MH  - Middle Aged
EDAT- 2003/12/31 05:00
MHDA- 2004/03/05 05:00
CRDT- 2003/12/31 05:00
PHST- 2003/12/31 05:00 [pubmed]
PHST- 2004/03/05 05:00 [medline]
PHST- 2003/12/31 05:00 [entrez]
AID - S0165460803002139 [pii]
AID - 10.1016/s0165-4608(03)00213-9 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2004 Jan 1;148(1):21-8. doi: 
      10.1016/s0165-4608(03)00213-9.