PMID- 14697639
OWN - NLM
STAT- MEDLINE
DCOM- 20040304
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 148
IP  - 1
DP  - 2004 Jan 1
TI  - Molecular cytogenetic characterization of rhabdomyosarcoma cell lines.
PG  - 35-43
AB  - Alveolar rhabdomyosarcomas (ARMS) are soft-tissue tumors that are genetically 
      characterized by the presence of reciprocal translocations that generate the 
      fusion gene PAX3-FOXO1A or PAX7-FOXO1A. For the study of the biologic 
      consequences of such rearrangements, several cell lines have been generated. 
      However, established cell lines accumulate chromosome and genetic aberrations 
      that make it difficult to draw significant conclusions. We have applied a set of 
      techniques that includes spectral karyotyping, fluorescence in situ hybridization 
      (FISH), comparative genomic hybridization (CGH), and microarray CGH, to the most 
      commonly used cell lines carrying the two fusion genes that are present in ARMS. 
      We have identified the bacterial artificial chromosomes that cover the 
      breakpoints at genes PAX3, PAX7, and FOXO1A, which can be used as FISH probes for 
      the translocations. The RH30 cell line, positive for the PAX3-FOXO1A fusion gene, 
      was found to be highly complex: wide range of chromosome number, more than 50 
      chromosome rearrangements, amplification of the hybrid gene, 24 DNA changes 
      detected by conventional CGH, and 21 gene copy changes detected by microarray CGH 
      (including several high-level amplifications). RMZ-RC2 cell line, positive for 
      the PAX7-FOXO1A, was in the near-tetraploid range with only nonclonal structural 
      rearrangements, amplification of the hybrid gene, 24 DNA changes by CGH, and 8 
      gene copy changes, confirming the previously reported high-level amplification of 
      MYCN.
FAU - Rodriguez-Perales, Sandra
AU  - Rodriguez-Perales S
AD  - Cytogenetics Unit, Department of Human Genetics, Centro Nacional de 
      Investigaciones Oncologicas, C/ Melchor Fernandez Almagro 3, 28029 Madrid, Spain.
FAU - Martinez-Ramirez, Angel
AU  - Martinez-Ramirez A
FAU - de Andres, Sara Alvarez
AU  - de Andres SA
FAU - Valle, Laura
AU  - Valle L
FAU - Urioste, Miguel
AU  - Urioste M
FAU - Benitez, Javier
AU  - Benitez J
FAU - Cigudosa, Juan C
AU  - Cigudosa JC
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - Cell Line, Tumor
MH  - *Chromosome Aberrations
MH  - Chromosome Banding
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Nucleic Acid Hybridization
MH  - Rhabdomyosarcoma/*genetics
MH  - Translocation, Genetic
EDAT- 2003/12/31 05:00
MHDA- 2004/03/05 05:00
CRDT- 2003/12/31 05:00
PHST- 2003/12/31 05:00 [pubmed]
PHST- 2004/03/05 05:00 [medline]
PHST- 2003/12/31 05:00 [entrez]
AID - S0165460803002164 [pii]
AID - 10.1016/s0165-4608(03)00216-4 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2004 Jan 1;148(1):35-43. doi: 
      10.1016/s0165-4608(03)00216-4.