PMID- 14698215
OWN - NLM
STAT- MEDLINE
DCOM- 20041025
LR  - 20190917
IS  - 0145-305X (Print)
IS  - 0145-305X (Linking)
VI  - 28
IP  - 4
DP  - 2004 Apr
TI  - Molecular cloning and characterization of tiger shrimp (Penaeus monodon) 
      transglutaminase.
PG  - 279-94
AB  - Transglutaminases (TG) are important for blood coagulation and post-translation 
      remodeling of proteins. Using a plaque screening assay, we isolated cDNA encoding 
      a novel TG from a shrimp (Penaeus monodon) hemocyte cDNA library. The TG cDNA 
      consists of 2988 bp with an open reading frame of 2271 bp. The deduced protein 
      has 757 amino acid residues, a calculated molecular mass of 84,713 Da and an 
      isoelectric point of 5.56. Neither a typical hydrophobic leader sequence nor a 
      transmembrane domain could be identified from the deduced sequence. Thus, shrimp 
      TG may be a typical cytoplasmic protein. The sequence of shrimp TG was similar to 
      crayfish, other invertebrate and vertebrate TG sequences. Enzyme activity was 
      detected in all organs tested. This is consistent with the widespread, low-level 
      expression of TG mRNA. However, high levels of TG expression were detected in 
      hematopoietic tissue. TG signals were stronger in mitotic cells, indicating that 
      cell proliferation and TG synthesis are associated. Preliminary data showed that 
      recombinant TG existed the enzyme activity but lacked coagulation activity.
FAU - Huang, Chih-Cheng
AU  - Huang CC
AD  - Institute of Zoology, National Taiwan University, Taipei 106, Taiwan, ROC.
FAU - Sritunyalucksana, Kallaya
AU  - Sritunyalucksana K
FAU - Soderhall, Kenneth
AU  - Soderhall K
FAU - Song, Yen-Ling
AU  - Song YL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Dev Comp Immunol
JT  - Developmental and comparative immunology
JID - 7708205
RN  - 0 (DNA, Complementary)
RN  - 0 (RNA, Messenger)
RN  - EC 2.3.2.13 (Transglutaminases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - DNA, Complementary/genetics
MH  - Hemocytes/enzymology
MH  - In Situ Hybridization
MH  - Molecular Sequence Data
MH  - Penaeidae/*enzymology/*genetics
MH  - Phylogeny
MH  - RNA, Messenger/genetics/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sequence Homology, Amino Acid
MH  - Transglutaminases/chemistry/*genetics
EDAT- 2003/12/31 05:00
MHDA- 2004/10/27 09:00
CRDT- 2003/12/31 05:00
PHST- 2003/12/31 05:00 [pubmed]
PHST- 2004/10/27 09:00 [medline]
PHST- 2003/12/31 05:00 [entrez]
AID - S0145305X03001691 [pii]
AID - 10.1016/j.dci.2003.08.005 [doi]
PST - ppublish
SO  - Dev Comp Immunol. 2004 Apr;28(4):279-94. doi: 10.1016/j.dci.2003.08.005.