PMID- 14698217
OWN - NLM
STAT- MEDLINE
DCOM- 20041025
LR  - 20190917
IS  - 0145-305X (Print)
IS  - 0145-305X (Linking)
VI  - 28
IP  - 4
DP  - 2004 Apr
TI  - Cloning and analyses of a BPI/LBP cDNA of the Atlantic cod (Gadus morhua L.).
PG  - 307-23
AB  - Using the differential screening technique, a cDNA related to the mammalian 
      family of lipid transfer/lipopolysaccharide-binding proteins was cloned from the 
      Atlantic cod (Gadus morhua L.). The gene is an ortholog of a recently identified 
      gene of rainbow trout (Oncorhynchus mykiss). Phylogenetic analyses suggest that 
      teleost BPI/LBP are modern descendants of the ancestor of mammalian 
      bactericidal/permeability-increasing protein (BPI) and lipopolysaccharide-binding 
      protein (LBP), and a gene of the urochordate Ciona intestinalis is related to 
      this gene family. Molecular modeling suggests that the structure of cod BPI/LBP 
      is similar to mammalian BPI and LBP, while its highly basic character is similar 
      to BPI. Cod BPI/LBP is constitutively expressed in head-kidney (HK) leukocytes. 
      After intraperitoneal injection of bacterin high levels of cod BPI/LBP mRNA were 
      detected also in peripheral blood cells and spleen, while moderate to low levels 
      of transcript were found in heart, liver, gills, skin, brain, and intestine. We 
      conclude that the patterns of charge and expression of cod BPI/LBP are more 
      similar to mammalian BPI than to mammalian LBP.
FAU - Stenvik, Jorgen
AU  - Stenvik J
AD  - Department of Marine Biotechnology, The Norwegian College of Fishery Science, 
      University of Tromso, N-9037 Tromso, Norway. joergens@nfh.uit.no
FAU - Solstad, Terese
AU  - Solstad T
FAU - Strand, Cato
AU  - Strand C
FAU - Leiros, Ingar
AU  - Leiros I
FAU - Jorgensen T, Trond O
AU  - Jorgensen T TO
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Dev Comp Immunol
JT  - Developmental and comparative immunology
JID - 7708205
RN  - 0 (Acute-Phase Proteins)
RN  - 0 (Antimicrobial Cationic Peptides)
RN  - 0 (Blood Proteins)
RN  - 0 (Carrier Proteins)
RN  - 0 (DNA, Complementary)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (bactericidal permeability increasing protein)
RN  - 0 (lipopolysaccharide-binding protein)
SB  - IM
MH  - Acute-Phase Proteins/chemistry/*genetics
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antimicrobial Cationic Peptides
MH  - Base Sequence
MH  - Blood Proteins/chemistry/*genetics
MH  - Carrier Proteins/chemistry/*genetics
MH  - Cloning, Molecular
MH  - DNA, Complementary/genetics
MH  - Fishes/*genetics/metabolism
MH  - Membrane Glycoproteins/chemistry/*genetics
MH  - Membrane Proteins/chemistry/*genetics
MH  - Models, Molecular
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - RNA, Messenger/genetics/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sequence Homology, Amino Acid
MH  - Static Electricity
EDAT- 2003/12/31 05:00
MHDA- 2004/10/27 09:00
CRDT- 2003/12/31 05:00
PHST- 2003/12/31 05:00 [pubmed]
PHST- 2004/10/27 09:00 [medline]
PHST- 2003/12/31 05:00 [entrez]
AID - S0145305X0300171X [pii]
AID - 10.1016/j.dci.2003.09.002 [doi]
PST - ppublish
SO  - Dev Comp Immunol. 2004 Apr;28(4):307-23. doi: 10.1016/j.dci.2003.09.002.