PMID- 14698755
OWN - NLM
STAT- MEDLINE
DCOM- 20040512
LR  - 20190712
IS  - 0306-4522 (Print)
IS  - 0306-4522 (Linking)
VI  - 123
IP  - 2
DP  - 2004
TI  - Prenatal cocaine exposure alters potassium-evoked dopamine release dynamics in 
      rat striatum.
PG  - 481-90
AB  - The emerging profile for the effects of prenatal cocaine exposure presents two 
      prominent features in the exposed offspring: cognitive/attention deficits and an 
      age-associated trend toward motor/tone abnormalities up to 2 years of age. One 
      candidate mechanism underlying these clinical features is long-lasting 
      alterations to dopamine (DA) neuron function. However, the impact of prenatal 
      cocaine exposure on DA release in dopaminergic terminal fields in vivo in mature 
      offspring is poorly understood. Long-Evans female rats were implanted with an 
      i.v. access port, bred, and given saline or cocaine-HCl (3 mg/kg/ml) for 
      gestational days (GD) 8-14 (1x/day), GD 15-21 (2x/day), or GD 8-21 (1x/day-GD 
      8-14, 2x/day-GD 15-21). Using in vivo high-speed chronoamperometric recordings, 
      potassium-stimulated DA release was measured in striatum of anesthetized male 
      offspring 90-150 days after birth. There was a trend toward increased 
      potassium-evoked DA signal amplitudes in offspring exposed to cocaine at any time 
      period examined. In offspring exposed to cocaine during GD 8-21 and GD 15-21, but 
      not at GD 8-14, there were significant decreases in the clearance capacity of the 
      potassium-evoked DA signal compared with control offspring. The time required to 
      clear 80% of the evoked DA signal (T(80)) in striatum for DA was significantly 
      prolonged (approximately 150% of control) and this effect was further increased 
      in the mean-evoked DA concentration range for these two groups. We also measured 
      total dopamine transporter (DAT) and tyrosine hydroxylase protein levels in these 
      offspring by blot immunolabeling and found a small, but significant, decrease in 
      DAT protein in striatum from offspring exposed at GD 8-21 and GD 15-21. 
      Collectively, these data demonstrate that prenatal cocaine exposure during 
      dopamine neuron neurogenesis has long-lasting effects on DA neuron function 
      lasting into early adulthood which may be related in part to steady state DAT 
      protein levels. These molecular events may be associated with established 
      cognitive deficits and perhaps the trends seen in altered motor behavior.
FAU - Salvatore, M F
AU  - Salvatore MF
AD  - Department of Anatomy and Neurobiology, University of Kentucky Medical Center, 
      Center for Sensor Technology, Davis Mills Building, 800 Rose Street, Lexington, 
      KY 40536, USA. msalv2@uky.edu
FAU - Hudspeth, O
AU  - Hudspeth O
FAU - Arnold, L E
AU  - Arnold LE
FAU - Wilson, P E
AU  - Wilson PE
FAU - Stanford, J A
AU  - Stanford JA
FAU - MacTutus, C F
AU  - MacTutus CF
FAU - Booze, R M
AU  - Booze RM
FAU - Gerhardt, G A
AU  - Gerhardt GA
LA  - eng
GR  - AG06434/AG/NIA NIH HHS/United States
GR  - AG13494/AG/NIA NIH HHS/United States
GR  - DA11337/DA/NIDA NIH HHS/United States
GR  - DA14944/DA/NIDA NIH HHS/United States
GR  - MH01245/MH/NIMH NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Neuroscience
JT  - Neuroscience
JID - 7605074
RN  - 0 (Dopamine Plasma Membrane Transport Proteins)
RN  - 0 (Dopamine Uptake Inhibitors)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Membrane Transport Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Slc6a3 protein, rat)
RN  - EC 1.14.16.2 (Tyrosine 3-Monooxygenase)
RN  - I5Y540LHVR (Cocaine)
RN  - RWP5GA015D (Potassium)
RN  - VTD58H1Z2X (Dopamine)
SB  - IM
MH  - Animals
MH  - Brain Chemistry/drug effects
MH  - Cocaine/*toxicity
MH  - Corpus Striatum/*drug effects/growth & development/metabolism
MH  - Dopamine/*metabolism
MH  - Dopamine Plasma Membrane Transport Proteins
MH  - Dopamine Uptake Inhibitors/*toxicity
MH  - Female
MH  - Immunoblotting
MH  - Immunohistochemistry
MH  - Male
MH  - *Membrane Glycoproteins
MH  - Membrane Transport Proteins/analysis/metabolism
MH  - Microelectrodes
MH  - *Nerve Tissue Proteins
MH  - Neurons/drug effects/metabolism
MH  - Potassium/pharmacology
MH  - Pregnancy
MH  - *Prenatal Exposure Delayed Effects
MH  - Rats
MH  - Tyrosine 3-Monooxygenase/analysis/metabolism
EDAT- 2003/12/31 05:00
MHDA- 2004/05/13 05:00
CRDT- 2003/12/31 05:00
PHST- 2003/12/31 05:00 [pubmed]
PHST- 2004/05/13 05:00 [medline]
PHST- 2003/12/31 05:00 [entrez]
AID - S0306452203007772 [pii]
AID - 10.1016/j.neuroscience.2003.10.002 [doi]
PST - ppublish
SO  - Neuroscience. 2004;123(2):481-90. doi: 10.1016/j.neuroscience.2003.10.002.