PMID- 14700733
OWN - NLM
STAT- MEDLINE
DCOM- 20040308
LR  - 20190701
IS  - 0304-3940 (Print)
IS  - 0304-3940 (Linking)
VI  - 354
IP  - 3
DP  - 2004 Jan 16
TI  - Temperature and 3,4-methylenedioxymethamphetamine alter human serotonin 
      transporter-mediated dopamine uptake.
PG  - 209-12
AB  - Although studies have suggested that dopamine can be transported by serotonin 
      transporters (SERTs), such activity has not been characterized at the cloned 
      SERTs. Dopamine and serotonin uptake by human SERT expressed in HEK-293 cells was 
      compared at 37 and 40 degrees C. Elevated temperature was found to alter 
      serotonin transport, but had no significant effect on dopamine transport. These 
      effects led to a 10-fold increase in the serotonin:dopamine transport ratio 
      reflecting an increased preference of SERTs for dopamine as opposed to serotonin 
      at the higher temperature. The effects of 3,4-methylenedioxymethamphetamine 
      (MDMA) on SERT-mediated dopamine transport were also evaluated by pre-incubating 
      SERT-expressing cells with MDMA. The presence of intracellular MDMA caused a 
      decrease in [3H]dopamine uptake but had no effect on [3H]serotonin transport 
      suggesting that intracellular MDMA may be capable of inhibiting transporter 
      function.
FAU - Saldana, Shannon N
AU  - Saldana SN
AD  - Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University 
      School of Pharmacy, MCMP RHPH 202, 575 Stadium Mall Drive, West Lafayette, IN 
      47907-2091, USA.
FAU - Barker, Eric L
AU  - Barker EL
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Ireland
TA  - Neurosci Lett
JT  - Neuroscience letters
JID - 7600130
RN  - 0 (Carrier Proteins)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Membrane Transport Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (SLC6A4 protein, human)
RN  - 0 (Serotonin Agents)
RN  - 0 (Serotonin Plasma Membrane Transport Proteins)
RN  - 333DO1RDJY (Serotonin)
RN  - KE1SEN21RM (N-Methyl-3,4-methylenedioxyamphetamine)
RN  - VTD58H1Z2X (Dopamine)
SB  - IM
MH  - Biological Transport/drug effects
MH  - Carrier Proteins/drug effects/*metabolism
MH  - Cell Line
MH  - Cells, Cultured
MH  - Dopamine/*metabolism
MH  - Embryo, Mammalian
MH  - Humans
MH  - Kidney
MH  - Membrane Glycoproteins/drug effects/*metabolism
MH  - *Membrane Transport Proteins
MH  - N-Methyl-3,4-methylenedioxyamphetamine/*pharmacology
MH  - *Nerve Tissue Proteins
MH  - Serotonin/metabolism
MH  - Serotonin Agents/*pharmacology
MH  - Serotonin Plasma Membrane Transport Proteins
MH  - *Temperature
MH  - Transfection/methods
EDAT- 2004/01/01 05:00
MHDA- 2004/03/09 05:00
CRDT- 2004/01/01 05:00
PHST- 2004/01/01 05:00 [pubmed]
PHST- 2004/03/09 05:00 [medline]
PHST- 2004/01/01 05:00 [entrez]
AID - S0304394003012254 [pii]
AID - 10.1016/j.neulet.2003.10.032 [doi]
PST - ppublish
SO  - Neurosci Lett. 2004 Jan 16;354(3):209-12. doi: 10.1016/j.neulet.2003.10.032.