PMID- 14702191
OWN - NLM
STAT- MEDLINE
DCOM- 20040830
LR  - 20170417
IS  - 0213-3911 (Print)
IS  - 0213-3911 (Linking)
VI  - 19
IP  - 1
DP  - 2004 Jan
TI  - Multicolor FISH probe sets and their applications.
PG  - 229-37
LID - 10.14670/HH-19.229 [doi]
AB  - Multicolor fluorescence in situ hybridization (FISH) assays are nowadays 
      indispensable for a precise description of complex chromosomal rearrangements. 
      Routine application of such techniques on human chromosomes started in 1996 with 
      the simultaneous use of all 24 human whole chromosome painting probes in 
      multiplex-FISH (M-FISH) and spectral karyotyping (SKY). Since then different 
      approaches for chromosomal differentiation based on multicolor-FISH (mFISH) 
      assays have been described. Predominantly, they have been established to 
      characterize marker chromosomes identified in conventional banding analysis. 
      Their characterization is of high clinical impact and is the requisite condition 
      for further molecular investigations aimed at the identification of 
      disease-related genes. Here we present a review on the available mFISH methods 
      including their advantages, limitations and possible applications.
FAU - Liehr, T
AU  - Liehr T
AD  - Institute of Human Genetics and Anthropology, Jena, Germany. 
      i8lith@mti.uni-jena.de
FAU - Starke, H
AU  - Starke H
FAU - Weise, A
AU  - Weise A
FAU - Lehrer, H
AU  - Lehrer H
FAU - Claussen, U
AU  - Claussen U
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
PL  - Spain
TA  - Histol Histopathol
JT  - Histology and histopathology
JID - 8609357
RN  - 0 (DNA Probes)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Genetic Markers)
RN  - 0 (Ligands)
SB  - IM
MH  - Animals
MH  - Centromere
MH  - Chromosome Banding
MH  - Chromosome Painting
MH  - Color
MH  - Cytogenetics/methods
MH  - DNA Probes
MH  - Female
MH  - Fluorescent Dyes
MH  - Genetic Markers
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Karyotyping
MH  - Ligands
MH  - Male
RF  - 71
EDAT- 2004/01/01 05:00
MHDA- 2004/08/31 05:00
CRDT- 2004/01/01 05:00
PHST- 2004/01/01 05:00 [pubmed]
PHST- 2004/08/31 05:00 [medline]
PHST- 2004/01/01 05:00 [entrez]
AID - 10.14670/HH-19.229 [doi]
PST - ppublish
SO  - Histol Histopathol. 2004 Jan;19(1):229-37. doi: 10.14670/HH-19.229.