PMID- 14704480
OWN - NLM
STAT- MEDLINE
DCOM- 20040610
LR  - 20151119
IS  - 1230-6002 (Print)
IS  - 1230-6002 (Linking)
VI  - 55
IP  - 5
DP  - 2003 Sep-Oct
TI  - MSRV pol sequence copy number as a potential marker of multiple sclerosis.
PG  - 869-75
AB  - Multiple sclerosis (MS) is a neurological disease in which demyelination in the 
      brain and spinal cord is observed. The causal influence of bacterial/viral 
      infections and genetic/immune factors in the etiology of multiple sclerosis is 
      suggested. Multiple sclerosis-related retrovirus (MSRV) is one of the potential 
      agents, which can lead to development of the disease. The aim of cytogenetic 
      studies was assessment of MSRV pol sequence copy number in patients with MS 
      compared to normal individuals. Cytogenetic slides with interphase nuclei and 
      extended chromatin fibers were prepared from peripheral blood of 16 patients with 
      MS and 10 healthy individuals. Fluorescence in situ hybridization (FISH) with 
      biotinylated product of polymerase chain reaction was used in order to analyze 
      MSRV pol sequence copy number in the examined material. Detection of MSRV pol 
      probe was carried out by immunological reaction with avidin-fluorescein and 
      biotinylated anti-avidin. MSRV pol sequence copy number was significantly greater 
      in MS patients than in normal individuals. Using FISH technique to extended 
      chromatin fibers, it was observed that MSRV pol exists as tandem repeats on 
      various chromosomes. The increased number of MSRV pol sequence has been found on 
      chromatin fibers of MS patients as compared to healthy controls.
FAU - Zawada, Mariola
AU  - Zawada M
AD  - Institute of Human Genetics, Polish Academy of Sciences, Strzeszynska 32, PL 
      60-479 Poznan, Poland.
FAU - Liwien, Izabela
AU  - Liwien I
FAU - Pernak, Monika
AU  - Pernak M
FAU - Januszkiewicz-Lewandowska, Danuta
AU  - Januszkiewicz-Lewandowska D
FAU - Nowicka-Kujawska, Karina
AU  - Nowicka-Kujawska K
FAU - Rembowska, Jolanta
AU  - Rembowska J
FAU - Lewandowski, Krzysztof
AU  - Lewandowski K
FAU - Hertmanowska, Hanna
AU  - Hertmanowska H
FAU - Wender, Mieczyslaw
AU  - Wender M
FAU - Nowak, Jerzy
AU  - Nowak J
LA  - eng
PT  - Journal Article
PL  - Poland
TA  - Pol J Pharmacol
JT  - Polish journal of pharmacology
JID - 9313882
RN  - 0 (Biomarkers)
RN  - 0 (Chromatin)
SB  - IM
MH  - Adult
MH  - *Biomarkers
MH  - Cell Nucleus/genetics/ultrastructure
MH  - Chromatin/genetics/ultrastructure
MH  - Endogenous Retroviruses/genetics/isolation & purification/pathogenicity
MH  - Gene Dosage
MH  - Genes, pol/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Interphase
MH  - Middle Aged
MH  - Multiple Sclerosis/*genetics/*physiopathology/virology
MH  - Polymerase Chain Reaction
MH  - Retroviridae/genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 2004/01/06 05:00
MHDA- 2004/06/21 10:00
CRDT- 2004/01/06 05:00
PHST- 2003/09/29 00:00 [received]
PHST- 2003/10/27 00:00 [revised]
PHST- 2004/01/06 05:00 [pubmed]
PHST- 2004/06/21 10:00 [medline]
PHST- 2004/01/06 05:00 [entrez]
PST - ppublish
SO  - Pol J Pharmacol. 2003 Sep-Oct;55(5):869-75.