PMID- 14704683
OWN - NLM
STAT- MEDLINE
DCOM- 20040907
LR  - 20220309
IS  - 1087-0156 (Print)
IS  - 1087-0156 (Linking)
VI  - 22
IP  - 2
DP  - 2004 Feb
TI  - Quantum dot ligands provide new insights into erbB/HER receptor-mediated signal 
      transduction.
PG  - 198-203
AB  - The erbB/HER family of transmembrane receptor tyrosine kinases (RTKs) mediate 
      cellular responses to epidermal growth factor (EGF) and related ligands. We have 
      imaged the early stages of RTK-dependent signaling in living cells using: (i) 
      stable expression of erbB1/2/3 fused with visible fluorescent proteins (VFPs), 
      (ii) fluorescent quantum dots (QDs) bearing epidermal growth factor (EGF-QD) and 
      (iii) continuous confocal laser scanning microscopy and flow cytometry. Here we 
      demonstrate that EGF-QDs are highly specific and potent in the binding and 
      activation of the EGF receptor (erbB1), being rapidly internalized into endosomes 
      that exhibit active trafficking and extensive fusion. EGF-QDs bound to erbB1 
      expressed on filopodia revealed a previously unreported mechanism of retrograde 
      transport to the cell body. When erbB2-monomeric yellow fluorescent protein 
      (mYFP) or erbB3-monomeric Citrine (mCitrine) were coexpressed with erbB1, the 
      rates and extent of endocytosis of EGF-QD and the RTK-VFP demonstrated that erbB2 
      but not erbB3 heterodimerizes with erbB1 after EGF stimulation, thereby 
      modulating EGF-induced signaling. QD-ligands will find widespread use in basic 
      research and biotechnological developments.
FAU - Lidke, Diane S
AU  - Lidke DS
AD  - Department of Molecular Biology, Max Planck Institute for Biophysical Chemistry, 
      Am Fassberg 11, D-37077 Gottingen, Germany. dlidke@gwdg.de
FAU - Nagy, Peter
AU  - Nagy P
FAU - Heintzmann, Rainer
AU  - Heintzmann R
FAU - Arndt-Jovin, Donna J
AU  - Arndt-Jovin DJ
FAU - Post, Janine N
AU  - Post JN
FAU - Grecco, Hernan E
AU  - Grecco HE
FAU - Jares-Erijman, Elizabeth A
AU  - Jares-Erijman EA
FAU - Jovin, Thomas M
AU  - Jovin TM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20040104
PL  - United States
TA  - Nat Biotechnol
JT  - Nature biotechnology
JID - 9604648
RN  - 0 (Oncogene Proteins v-erbB)
RN  - 0 (Receptors, Cell Surface)
RN  - 62229-50-9 (Epidermal Growth Factor)
SB  - IM
CIN - Nat Biotechnol. 2004 Feb;22(2):169-70. PMID: 14755288
MH  - Animals
MH  - Cell Membrane/*metabolism
MH  - Cricetinae
MH  - Endosomes/metabolism/ultrastructure
MH  - Epidermal Growth Factor/metabolism
MH  - Humans
MH  - Motion
MH  - Oncogene Proteins v-erbB/metabolism
MH  - Protein Binding
MH  - Protein Interaction Mapping/*methods
MH  - Protein Transport/*physiology
MH  - *Quantum Dots
MH  - Receptors, Cell Surface/*metabolism
MH  - Signal Transduction/*physiology
MH  - Spectrometry, Fluorescence/*methods
EDAT- 2004/01/06 05:00
MHDA- 2004/09/08 05:00
CRDT- 2004/01/06 05:00
PHST- 2003/10/24 00:00 [received]
PHST- 2003/11/24 00:00 [accepted]
PHST- 2004/01/06 05:00 [pubmed]
PHST- 2004/09/08 05:00 [medline]
PHST- 2004/01/06 05:00 [entrez]
AID - nbt929 [pii]
AID - 10.1038/nbt929 [doi]
PST - ppublish
SO  - Nat Biotechnol. 2004 Feb;22(2):198-203. doi: 10.1038/nbt929. Epub 2004 Jan 4.