PMID- 14705012
OWN - NLM
STAT- MEDLINE
DCOM- 20040803
LR  - 20191210
IS  - 0006-3592 (Print)
IS  - 0006-3592 (Linking)
VI  - 85
IP  - 1
DP  - 2004 Jan 5
TI  - Enhanced biological phosphorus removal in a sequencing batch reactor using 
      propionate as the sole carbon source.
PG  - 56-67
AB  - An enhanced biological phosphorus removal (EBPR) system was developed in a 
      sequencing batch reactor (SBR) using propionate as the sole carbon source. The 
      microbial community was followed using fluorescence in situ hybridization (FISH) 
      techniques and Candidatus 'Accumulibacter phosphatis' were quantified from the 
      start up of the reactor until steady state. A series of SBR cycle studies was 
      performed when 55% of the SBR biomass was Accumulibacter, a confirmed 
      polyphosphate accumulating organism (PAO) and when Candidatus 'Competibacter 
      phosphatis', a confirmed glycogen-accumulating organism (GAO), was essentially 
      undetectable. These experiments evaluated two different carbon sources 
      (propionate and acetate), and in every case, two different P-release rates were 
      detected. The highest rate took place while there was volatile fatty acid (VFA) 
      in the mixed liquor, and after the VFA was depleted a second P-release rate was 
      observed. This second rate was very similar to the one detected in experiments 
      performed without added VFA.A kinetic and stoichiometric model developed as a 
      modification of Activated Sludge Model 2 (ASM2) including glycogen economy, was 
      fitted to the experimental profiles. The validation and calibration of this model 
      was carried out with the cycle study experiments performed using both VFAs. The 
      effect of pH from 6.5 to 8.0 on anaerobic P-release and VFA-uptake and aerobic 
      P-uptake was also studied using propionate. The optimal overall working pH was 
      around 7.5. This is the first study of the microbial community involved in EBPR 
      developed with propionate as a sole carbon source along with detailed process 
      performance investigations of the propionate-utilizing PAOs.
CI  - Copyright 2003 Wiley Periodicals, Inc.
FAU - Pijuan, M
AU  - Pijuan M
AD  - Departament d'Enginyeria Quimica, ETSE, Universitat Autonoma de Barcelona, 08193 
      Bellaterra (Barcelona), Spain.
FAU - Saunders, A M
AU  - Saunders AM
FAU - Guisasola, A
AU  - Guisasola A
FAU - Baeza, J A
AU  - Baeza JA
FAU - Casas, C
AU  - Casas C
FAU - Blackall, L L
AU  - Blackall LL
LA  - eng
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Validation Study
PL  - United States
TA  - Biotechnol Bioeng
JT  - Biotechnology and bioengineering
JID - 7502021
RN  - 0 (Industrial Waste)
RN  - 0 (Propionates)
RN  - 0 (Sewage)
RN  - 0 (Water Pollutants, Chemical)
RN  - 27YLU75U4W (Phosphorus)
RN  - EC 6.2.1.1 (Acetate-CoA Ligase)
SB  - IM
MH  - Acetate-CoA Ligase/*metabolism
MH  - Bioreactors/*microbiology
MH  - Computer Simulation
MH  - Industrial Waste/*prevention & control
MH  - *Models, Biological
MH  - Phosphorus/pharmacokinetics
MH  - Propionates/*metabolism
MH  - Sewage/*microbiology
MH  - Water Pollutants, Chemical/pharmacokinetics
MH  - Water Purification/methods
EDAT- 2004/01/06 05:00
MHDA- 2004/08/04 05:00
CRDT- 2004/01/06 05:00
PHST- 2004/01/06 05:00 [pubmed]
PHST- 2004/08/04 05:00 [medline]
PHST- 2004/01/06 05:00 [entrez]
AID - 10.1002/bit.10813 [doi]
PST - ppublish
SO  - Biotechnol Bioeng. 2004 Jan 5;85(1):56-67. doi: 10.1002/bit.10813.