PMID- 14705986
OWN - NLM
STAT- MEDLINE
DCOM- 20040826
LR  - 20231213
IS  - 0001-2815 (Print)
IS  - 0001-2815 (Linking)
VI  - 63
IP  - 2
DP  - 2004 Feb
TI  - Differential up-regulation of HLA-DM, invariant chain, and CD83 on myeloid and 
      plasmacytoid dendritic cells from peripheral blood.
PG  - 149-57
AB  - Two main dendritic cell (DC) subsets have been described in peripheral blood, the 
      myeloid subset or DC1 that is characterized by the presence of CD11c and the 
      plasmacytoid subset or DC2 negative for this marker. The two subsets may perform 
      different functions and have been defined as immunogenic (the myeloid subset) or 
      tolerogenic (the plasmacytoid subset). The expression of human leukocyte antigen 
      (HLA)-DM molecules, which act as peptide editors in the antigen presentation 
      process, was studied in freshly isolated plasmacytoid and myeloid DCs from 
      peripheral blood. The expression of the invariant chain (Ii), the major 
      histocompatibility complex class II (MHC-II) : class II-associated Ii peptide 
      (CLIP) complex, and CD83 was also investigated. The results showed that 
      intracellular expression of HLA-DM and the Ii was significantly higher in the 
      plasmacytoid than in the myeloid DC subset. In contrast, a higher fraction of 
      cell expressing MHC-II : CLIP complex was found in the myeloid than in the 
      plasmacytoid DC subpopulation. CD83 was not detected in any of these two subsets. 
      Following culture of these cells with interleukin-3 (IL-3), tumor necrosis 
      factor-alpha (TNFalpha) and/or heat shock protein-70 (HSP-70), the expression of 
      intracellular HLA-DM was up-regulated in the myeloid DCs to levels similar to 
      those found in the plasmacytoid DCs, whilst the Ii was down-regulated in the 
      plasmacytoid subset to similar levels to those expressed in the myeloid DCs. In 
      addition, CD83 was up-regulated in the myeloid (CD11c+) but not in the 
      plasmacytoid (CD11c-) DCs. The expression pattern of these antigen-processing 
      molecules could be related to the immaturity and function attributed to these DC 
      subsets.
FAU - Gomez, J
AU  - Gomez J
AD  - Histocompatibility and Immunogenetics Research Group, National Blood Service, 
      Colindale Avenue, London, UK.
FAU - Borras, F E
AU  - Borras FE
FAU - Singh, R
AU  - Singh R
FAU - Rajananthanan, P
AU  - Rajananthanan P
FAU - English, N
AU  - English N
FAU - Knight, S C
AU  - Knight SC
FAU - Navarrete, C V
AU  - Navarrete CV
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Tissue Antigens
JT  - Tissue antigens
JID - 0331072
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Differentiation, B-Lymphocyte)
RN  - 0 (Antineoplastic Agents)
RN  - 0 (HLA-D Antigens)
RN  - 0 (HLA-DM antigens)
RN  - 0 (HSP70 Heat-Shock Proteins)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (Immunoglobulins)
RN  - 0 (Interleukin-3)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Peptide Fragments)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (invariant chain)
SB  - IM
MH  - Antigen Presentation
MH  - Antigens, CD
MH  - Antigens, Differentiation, B-Lymphocyte/immunology/*metabolism
MH  - Antineoplastic Agents/pharmacology
MH  - Dendritic Cells/*immunology
MH  - Genes, MHC Class II/physiology
MH  - HLA-D Antigens/immunology/*metabolism
MH  - HSP70 Heat-Shock Proteins/pharmacology
MH  - Histocompatibility Antigens Class II/immunology/*metabolism
MH  - Humans
MH  - Immunoglobulins/immunology/*metabolism
MH  - Interleukin-3/pharmacology
MH  - Leukocytes, Mononuclear/cytology/immunology/metabolism
MH  - Membrane Glycoproteins/immunology/*metabolism
MH  - Myeloid Cells/cytology/*immunology
MH  - Peptide Fragments/chemistry/genetics/metabolism
MH  - Plasma Cells/cytology/*immunology
MH  - Tumor Necrosis Factor-alpha/pharmacology
MH  - Up-Regulation
MH  - CD83 Antigen
EDAT- 2004/01/07 05:00
MHDA- 2004/08/27 05:00
CRDT- 2004/01/07 05:00
PHST- 2004/01/07 05:00 [pubmed]
PHST- 2004/08/27 05:00 [medline]
PHST- 2004/01/07 05:00 [entrez]
AID - 159 [pii]
AID - 10.1111/j.1399-0039.2004.00159.x [doi]
PST - ppublish
SO  - Tissue Antigens. 2004 Feb;63(2):149-57. doi: 10.1111/j.1399-0039.2004.00159.x.