PMID- 14706153
OWN - NLM
STAT- MEDLINE
DCOM- 20040430
LR  - 20191210
IS  - 1009-2137 (Print)
IS  - 1009-2137 (Linking)
VI  - 11
IP  - 6
DP  - 2003 Dec
TI  - [Effects of leukocyte elimination before storage on quality of red blood cell 
      concentration].
PG  - 650-3
AB  - The objective of this study was to explore the possible effects of leukocyte 
      elimination by filteration before storage on the quality of red blood cell 
      concentrations (RCC) that prepared through two procedures. Eight units of red 
      blood cell concentrations derived from whole blood after plasma separated (RCC1) 
      and eight units of red blood cell concentrations derived from whole blood after 
      platelet-rich plasma separated (RCC2) were divided randomly into filtered group 
      and control group respectively. The RCC of filtered group were filtered by 
      leukocyte deplete filter before storage. The control group didn't have any other 
      treatments. These two groups were stored for five weeks at 4 degrees C according 
      to AABB standard. Mean corpuscular volume (MCV), mean corpuscular hemoglobin 
      (MCH), mean corpuscular hemoglobin concentration (MCHC) and plasma concentration 
      of K(+) and lactate dehydrogenase (LDH), free hemoglobin (FHb), adenosine 
      triphosphate (ATP) of red blood cell of all RCC were evaluated weekly, and 
      bacteria contamination of all RCC was also detected after five weeks of storage. 
      The results showed that there was no difference of MCV, MCH and MCHC and ATP 
      level of red blood cell in all RCC of two groups, the ATP of red blood cell was 
      lower than the control group on week 4 and 5. The average concentration of K(+) 
      of the filtered group was less than the control group. The differences are 
      significant except that of RCC1 stored till the third week. The plasma LDH 
      concentration of filtered group was less than the control group, and the 
      differences were exacerbate during the storing time prolonged. FHb release in the 
      filtered group of RCC2 was significant less than that of control, but no 
      significant difference was found between the two groups of RCC1. It was concluded 
      that leukocyte elimination by filter before storage could be benefit to RCC 
      preservation.
FAU - Wang, Hai-Bao
AU  - Wang HB
AD  - Department of blood Transfusion, General Hospital of PLA, Beijing 100853, China. 
      wanghb9893@sina.com
FAU - Liu, Jing-Han
AU  - Liu JH
FAU - Lin, Zi-Lin
AU  - Lin ZL
FAU - Li, Xi-Jin
AU  - Li XJ
FAU - Lu, Liu-Cai
AU  - Lu LC
FAU - Ouyang, Xi-Lin
AU  - Ouyang XL
FAU - Huang, Ning
AU  - Huang N
LA  - chi
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhongguo Shi Yan Xue Ye Xue Za Zhi
JT  - Zhongguo shi yan xue ye xue za zhi
JID - 101084424
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - EC 1.1.1.27 (L-Lactate Dehydrogenase)
RN  - RWP5GA015D (Potassium)
SB  - IM
MH  - Adenosine Triphosphate/metabolism
MH  - Blood Component Removal
MH  - *Blood Preservation
MH  - Erythrocytes/*physiology
MH  - Filtration
MH  - Humans
MH  - L-Lactate Dehydrogenase/blood
MH  - Leukocytes
MH  - Potassium/blood
EDAT- 2004/01/07 05:00
MHDA- 2004/05/01 05:00
CRDT- 2004/01/07 05:00
PHST- 2004/01/07 05:00 [pubmed]
PHST- 2004/05/01 05:00 [medline]
PHST- 2004/01/07 05:00 [entrez]
AID - 1009-2137(2003)06-0650-04 [pii]
PST - ppublish
SO  - Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2003 Dec;11(6):650-3.