PMID- 14706284
OWN - NLM
STAT- MEDLINE
DCOM- 20040917
LR  - 20220224
IS  - 0143-4160 (Print)
IS  - 0143-4160 (Linking)
VI  - 35
IP  - 2
DP  - 2004 Feb
TI  - A role for hTRPC1 and lipid raft domains in store-mediated calcium entry in human 
      platelets.
PG  - 107-13
AB  - We have previously suggested that store-mediated Ca2+ entry (SMCE) in human 
      platelets may be activated by a secretion-like coupling model, involving de novo 
      coupling of the type II inositol 1,4,5-trisphosphate receptor (IP(3)RII) to the 
      putative Ca2+ entry channel, hTRPC1. In other cells, hTRPC1 has been reported to 
      be associated with cholesterol-rich lipid raft domains (LRDs) in the plasma 
      membrane. Here we have shown that hTRPC1 is largely associated with 
      detergent-resistant platelet membranes, from which it is partially released when 
      the cells are depleted of cholesterol by treatment with methyl-beta-cyclodextrin 
      (MBCD). MBCD treatment inhibited thapsigargin (TG)-evoked SMCE in a 
      concentration-dependent manner, reducing it to 38.1+/-4.1% at a concentration of 
      10mM. Similarly, the Ca2+ entry evoked by thrombin (1unit/ml) was reduced to 
      48.2+/-4.5% of control following MBCD (10mM) treatment. Thrombin- and TG-evoked 
      coupling between IP(3)RII and hTRPC1 was also reduced following cholesterol 
      depletion. These results suggest that hTRPC1 is associated with LRDs in human 
      platelets and that these domains are important for its participation in SMCE.
FAU - Brownlow, Sharon L
AU  - Brownlow SL
AD  - Department of Physiology, University of Cambridge, Downing Street, Cambridge CB2 
      3EG, UK.
FAU - Harper, Alan G S
AU  - Harper AG
FAU - Harper, Matthew T
AU  - Harper MT
FAU - Sage, Stewart O
AU  - Sage SO
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Cell Calcium
JT  - Cell calcium
JID - 8006226
RN  - 0 (Calcium Channels)
RN  - 0 (Cyclodextrins)
RN  - 0 (TRPC Cation Channels)
RN  - 0 (beta-Cyclodextrins)
RN  - 0 (methyl-beta-cyclodextrin)
RN  - 0 (transient receptor potential cation channel, subfamily C, member 1)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Blood Platelets/drug effects/*metabolism
MH  - Calcium/*metabolism
MH  - Calcium Channels/blood/*physiology
MH  - Cyclodextrins/pharmacology
MH  - Dose-Response Relationship, Drug
MH  - Humans
MH  - Membrane Microdomains/drug effects/*physiology
MH  - TRPC Cation Channels
MH  - *beta-Cyclodextrins
EDAT- 2004/01/07 05:00
MHDA- 2004/09/21 05:00
CRDT- 2004/01/07 05:00
PHST- 2004/01/07 05:00 [pubmed]
PHST- 2004/09/21 05:00 [medline]
PHST- 2004/01/07 05:00 [entrez]
AID - S0143416003001878 [pii]
AID - 10.1016/j.ceca.2003.08.002 [doi]
PST - ppublish
SO  - Cell Calcium. 2004 Feb;35(2):107-13. doi: 10.1016/j.ceca.2003.08.002.