PMID- 14707431
OWN - NLM
STAT- MEDLINE
DCOM- 20041028
LR  - 20131121
IS  - 0006-3126 (Print)
IS  - 0006-3126 (Linking)
VI  - 85
IP  - 3
DP  - 2004
TI  - Glutathione recycling and antioxidant enzyme activities in erythrocytes of term 
      and preterm newborns at birth.
PG  - 188-94
AB  - We previously demonstrated a high susceptibility of neonatal red blood cells 
      (RBC) to oxidative stress at birth. The aim of this study was to compare the RBC 
      antioxidant capacity and redox cycle enzyme activities as well as glutathione 
      (GSH) recycling in full-term and preterm infants at birth and in normal adults. 
      GSH and GSH disulfide (GSSG) concentrations, GSH/GSSG ratio, and the activities 
      of glucose-6-phosphate dehydrogenase (G-6-PDH), GSH peroxidase, GSH reductase 
      (GR), catalase (CAT), superoxide dismutase (SOD), and hexokinase (HK) were 
      measured in RBC of 25 healthy adults and 56 newborns (23 term, 33 preterm) at 
      birth. The GSH recycling was measured in adult and newborn RBC exposed to 
      oxidative stress (1 mM tert-butylhydroperoxide). The RBC of term and preterm 
      babies showed higher GSH, GSSG, G-6-PDH, GR, and HK levels/activities and lower 
      GSH/GSSG ratios and higher GSH-recycling rates than those of adults. In preterm 
      babies significant correlations were found between G-6-PDH and CAT, GSH, GSH/GSSG 
      ratio, and GSSG (r = -0.67, r = 0.71, r = -0.66, p < 0.01; r = 0.71, p < 0.05, 
      respectively). In term newborns, statistically significant correlations were 
      observed between G-6-PDH and CAT, SOD, and GSH (r = -0.65, r = -0.65, r = -0.69, 
      p < 0.01, respectively). The results indicate the central role of the G-6-PDH 
      activity in antioxidant defenses. We speculate that preterm babies have prompter 
      involvement of antioxidant defenses than term babies.
CI  - Copyright 2004 S. Karger AG, Basel
FAU - Frosali, Simona
AU  - Frosali S
AD  - Department of Neuroscience, Pharmacology Unit, University of Siena, Siena, Italy.
FAU - Di Simplicio, Paolo
AU  - Di Simplicio P
FAU - Perrone, Serafina
AU  - Perrone S
FAU - Di Giuseppe, Danila
AU  - Di Giuseppe D
FAU - Longini, Mariangela
AU  - Longini M
FAU - Tanganelli, Donatella
AU  - Tanganelli D
FAU - Buonocore, Giuseppe
AU  - Buonocore G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20031224
PL  - Switzerland
TA  - Biol Neonate
JT  - Biology of the neonate
JID - 0247551
RN  - EC 1.1.1.49 (Glucosephosphate Dehydrogenase)
RN  - EC 1.11.1.6 (Catalase)
RN  - EC 1.11.1.9 (Glutathione Peroxidase)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - EC 1.8.1.7 (Glutathione Reductase)
RN  - EC 2.7.1.1 (Hexokinase)
RN  - GAN16C9B8O (Glutathione)
RN  - ULW86O013H (Glutathione Disulfide)
SB  - IM
MH  - Adult
MH  - Catalase/blood
MH  - Erythrocytes/*enzymology/metabolism
MH  - Glucosephosphate Dehydrogenase/blood
MH  - Glutathione/*blood
MH  - Glutathione Disulfide/*blood
MH  - Glutathione Peroxidase/blood
MH  - Glutathione Reductase/blood
MH  - Hexokinase/blood
MH  - Humans
MH  - Infant, Newborn/*blood
MH  - Infant, Premature/*blood
MH  - Middle Aged
MH  - Oxidation-Reduction
MH  - Oxidative Stress/physiology
MH  - Superoxide Dismutase/blood
EDAT- 2004/01/07 05:00
MHDA- 2004/10/29 09:00
CRDT- 2004/01/07 05:00
PHST- 2003/02/07 00:00 [received]
PHST- 2003/10/16 00:00 [accepted]
PHST- 2004/01/07 05:00 [pubmed]
PHST- 2004/10/29 09:00 [medline]
PHST- 2004/01/07 05:00 [entrez]
AID - 75814 [pii]
AID - 10.1159/000075814 [doi]
PST - ppublish
SO  - Biol Neonate. 2004;85(3):188-94. doi: 10.1159/000075814. Epub 2003 Dec 24.