PMID- 14707467
OWN - NLM
STAT- MEDLINE
DCOM- 20040210
LR  - 20171101
IS  - 1018-2438 (Print)
IS  - 1018-2438 (Linking)
VI  - 132
IP  - 4
DP  - 2003 Dec
TI  - Gene expression analysis of atopic dermatitis-like skin lesions induced in NC/Nga 
      mice by mite antigen stimulation under specific pathogen-free conditions.
PG  - 355-63
AB  - BACKGROUND: Atopic dermatitis (AD) is a chronic relapsing inflammation 
      characterized by pruritic and eczematous skin lesions usually observed in 
      patients with a familial history of atopic diseases, but its exact etiology is 
      unclear. An animal model is indispensable for the analysis of the pathogenesis 
      and the development of new drugs to treat this disease. Here, we compare changes 
      in gene expression profiles in the AD-like skin lesions of NC/Nga or BALB/c mice 
      stimulated intradermally by mite antigen under specific pathogen-free (SPF) 
      conditions. METHODS: Mite Extract-Dp was injected intradermally into the right 
      and left pinnae and into the skin of the back of NC/Nga or BALB/c mice in 2 
      places once per 3 days, and the clinical symptoms and the ear thickness were 
      measured. On day 14 or day 28 after starting mite extract injection, we collected 
      plasma and pinnae from NC/Nga or BALB/c mice. The amount of total immunoglobulin 
      E (IgE) in plasma was assayed. We analyzed mRNA transcripts in pinnae using 
      real-time quantitative PCR for the murine counterparts of several known 
      allergy-related genes. Moreover, genome-wide gene expression in pinnae from 
      NC/Nga mice was analyzed using high-density oligonucleotide arrays (GeneChip, 
      Affymetrix). RESULTS: From 2 weeks after stimulation, marked skin inflammation 
      was induced in the pinnae of NC/Nga but not BALB/c mice. However, IgE levels in 
      sera rose equally in both strains. Quantitative PCR analysis and comprehensive 
      GeneChip analysis of the AD-like pinna skin lesions revealed that their 
      development was accompanied by changes in expression of more than 1,000 genes. 
      These included cytokines, cytokine receptors, proteases, and adhesion molecules. 
      Furthermore, genes thus far not reported in association with AD were also 
      affected. CONCLUSION: From these results, the NC/Nga mouse model using mite 
      sensitization under SPF conditions could be useful for elucidating the mechanisms 
      of AD pathogenesis and developing more effective therapy for AD.
CI  - Copyright 2003 S. Karger AG, Basel
FAU - Heishi, Masayuki
AU  - Heishi M
AD  - Genox Research, Inc., Tsukuba-shi, Japan.
FAU - Imai, Yukiho
AU  - Imai Y
FAU - Katayama, Hiroko
AU  - Katayama H
FAU - Hashida, Ryoichi
AU  - Hashida R
FAU - Ito, Mikito
AU  - Ito M
FAU - Shinagawa, Akira
AU  - Shinagawa A
FAU - Sugita, Yuji
AU  - Sugita Y
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Int Arch Allergy Immunol
JT  - International archives of allergy and immunology
JID - 9211652
RN  - 0 (Antigens, Dermatophagoides)
RN  - 0 (Arthropod Proteins)
RN  - 0 (Ccl11 protein, mouse)
RN  - 0 (Chemokine CCL11)
RN  - 0 (Chemokine CCL5)
RN  - 0 (Chemokines, CC)
RN  - 0 (Dermatophagoides pteronyssinus antigen p 2)
RN  - 0 (Interleukin-18)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 63231-63-0 (RNA)
SB  - IM
MH  - Animals
MH  - Antigens, Dermatophagoides/*immunology
MH  - Arthropod Proteins
MH  - Chemokine CCL11
MH  - Chemokine CCL5/genetics/immunology
MH  - Chemokines, CC/genetics/immunology
MH  - Dermatitis, Atopic/genetics/*immunology/pathology
MH  - Ear/pathology
MH  - Edema/immunology/pathology
MH  - Gene Expression Regulation/*immunology
MH  - Immunoglobulin E/blood
MH  - Interleukin-18/genetics/immunology
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mites/immunology
MH  - Oligonucleotide Array Sequence Analysis
MH  - RNA/chemistry/genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Specific Pathogen-Free Organisms
EDAT- 2004/01/07 05:00
MHDA- 2004/02/11 05:00
CRDT- 2004/01/07 05:00
PHST- 2003/01/20 00:00 [received]
PHST- 2003/09/24 00:00 [accepted]
PHST- 2004/01/07 05:00 [pubmed]
PHST- 2004/02/11 05:00 [medline]
PHST- 2004/01/07 05:00 [entrez]
AID - 74903 [pii]
AID - 10.1159/000074903 [doi]
PST - ppublish
SO  - Int Arch Allergy Immunol. 2003 Dec;132(4):355-63. doi: 10.1159/000074903.