PMID- 14708752
OWN - NLM
STAT- MEDLINE
DCOM- 20040210
LR  - 20230320
IS  - 0095-3628 (Print)
IS  - 0095-3628 (Linking)
VI  - 46
IP  - 2
DP  - 2003 Aug
TI  - Dynamics of microcystin-degrading bacteria in mucilage of Microcystis.
PG  - 279-88
AB  - To reveal the process of degradation of hepatotoxic microcystin produced in 
      Microcystis cells during the Microcystis bloom period, we used fluorescence in 
      situ hybridization (FISH) to analyze the population dynamics of 
      microcystin-degrading bacteria in Microcystis mucilage. We designed and applied 
      an oligonucleotide probe targeted to the 16S rRNA sequence of strain Y2 of a 
      microcystin-degrading bacterium (MCD-bacterium), which was isolated from Lake 
      Suwa, Japan. In both the 1998 and 1999 tests, FISH clearly showed that 
      MCD-bacteria existed in the mucilage and that, when a high concentration of 
      cell-bound microcystin was detected, MCD-bacteria exceeded 10% of the sum of 
      bacteria hybridized with group-specific probes. The concentration of MCD-bacteria 
      was highest in summer 1998, when a toxic species, M. viridis, was dominant. There 
      was a high correlation between the number of MCD-bacteria in the mucilage and the 
      concentration of cell-bound microcystin in the lake. Our results suggest that 
      MCD-bacteria responded to changes in the concentration of microcystin and 
      degraded the microcystin when it was released from Microcystis cells. We also 
      analyzed changes in the bacterial community structure associated with the 
      Microcystis colonies by using domain- and group-specific oligonucleotide probes. 
      Changes in the concentrations of the Cytophaga/Flavobacterium group and 
      delta-Proteobacteria, which can degrade macromolecules derived from Microcystis 
      cells, were synchronized with changes in the concentration of Microcystis. The 
      results not only suggest the significant role of MCD-bacteria in detoxification, 
      but also demonstrate a possible sequence of degradation from Microcystis cells to 
      microcystin maintained in the cell, which is then carried out by bacterial 
      consortia in the mucilage.
FAU - Maruyama, T
AU  - Maruyama T
AD  - Department of Biology and Geosciences, Faculty of Science, Shizuoka University, 
      Shizuoka 422-8529, Japan.
FAU - Kato, K
AU  - Kato K
FAU - Yokoyama, A
AU  - Yokoyama A
FAU - Tanaka, T
AU  - Tanaka T
FAU - Hiraishi, A
AU  - Hiraishi A
FAU - Park, H D
AU  - Park HD
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Microb Ecol
JT  - Microbial ecology
JID - 7500663
RN  - 0 (Adhesives)
RN  - 0 (Carcinogens)
RN  - 0 (Microcystins)
RN  - 0 (Peptides, Cyclic)
RN  - 77238-39-2 (microcystin)
SB  - IM
MH  - Adhesives
MH  - Carcinogens/*metabolism
MH  - Ecology
MH  - In Situ Hybridization, Fluorescence
MH  - Microcystins
MH  - Microcystis/genetics/*physiology
MH  - Peptides, Cyclic/*metabolism
MH  - Population Dynamics
MH  - *Water Microbiology
MH  - Water Supply
EDAT- 2004/01/08 05:00
MHDA- 2004/02/11 05:00
CRDT- 2004/01/08 05:00
PHST- 2004/01/08 05:00 [pubmed]
PHST- 2004/02/11 05:00 [medline]
PHST- 2004/01/08 05:00 [entrez]
AID - 10.1007/s00248-002-3007-7 [doi]
PST - ppublish
SO  - Microb Ecol. 2003 Aug;46(2):279-88. doi: 10.1007/s00248-002-3007-7.