PMID- 14709039
OWN - NLM
STAT- MEDLINE
DCOM- 20040408
LR  - 20220317
IS  - 0002-7863 (Print)
IS  - 0002-7863 (Linking)
VI  - 126
IP  - 1
DP  - 2004 Jan 14
TI  - In vitro evolution of functional DNA using capillary electrophoresis.
PG  - 20-1
AB  - Electrophoretic selection with capillary electrophoresis (CE) is used, for the 
      first time, to isolate functional nucleic acid sequences using SELEX (systematic 
      evolution of ligands by exponential enrichment). SELEX uses molecular evolution 
      to select functional sequences (aptamers) from random RNA or DNA libraries. 
      Conventional SELEX is usually performed with affinity chromatography, which may 
      introduce significant bias into the selection step. Important biases include the 
      slow kinetics involved in the elution of strongly bound sequences and performing 
      the selection with the target molecule tethered to the stationary support, not in 
      free solution. In this novel CE-SELEX approach, selection occurs in free 
      solution. The nucleic acid sequences that bind the target undergo a mobility 
      shift, migrating at a different rate, allowing them to be separated from the 
      inactive sequences. Thus, there is no need to wash the active sequences off a 
      column as in conventional SELEX, eliminating any kinetic bias. In this work, the 
      viability of CE-SELEX was demonstrated by performing selections against 
      immunoglobulin E (IgE). Anti-IgE aptamers with dissociation constants as low as 
      40 nM were obtained in only two rounds of selection.
FAU - Mendonsa, Shaun D
AU  - Mendonsa SD
AD  - Department of Chemistry, University of Minnesota, 207 Pleasant Street SE, 
      Minneapolis, Minnesota 55455, USA.
FAU - Bowser, Michael T
AU  - Bowser MT
LA  - eng
GR  - GM63533/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Am Chem Soc
JT  - Journal of the American Chemical Society
JID - 7503056
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - DNA/*chemistry/immunology/metabolism
MH  - Electrophoresis, Capillary/*methods
MH  - Humans
MH  - Immunoglobulin E/chemistry/immunology/metabolism
MH  - Kinetics
MH  - Mice
EDAT- 2004/01/08 05:00
MHDA- 2004/04/09 05:00
CRDT- 2004/01/08 05:00
PHST- 2004/01/08 05:00 [pubmed]
PHST- 2004/04/09 05:00 [medline]
PHST- 2004/01/08 05:00 [entrez]
AID - 10.1021/ja037832s [doi]
PST - ppublish
SO  - J Am Chem Soc. 2004 Jan 14;126(1):20-1. doi: 10.1021/ja037832s.