PMID- 14709246
OWN - NLM
STAT- MEDLINE
DCOM- 20040412
LR  - 20071114
IS  - 0889-2229 (Print)
IS  - 0889-2229 (Linking)
VI  - 19
IP  - 12
DP  - 2003 Dec
TI  - Peripheral blood Dendritic cells are not a major reservoir for HIV type 1 in 
      infected individuals on virally suppressive HAART.
PG  - 1097-103
AB  - Dendritic cells (DCs) are potent antigen-presenting cells, and their 
      physiological localization in tissues that interact with the external environment 
      is important as a first barrier against pathogens such as human immunodeficiency 
      virus type I (HIV-1). Several models have been proposed to explain the possible 
      role of DCs as a reservoir for HIV-1 in patients on virally suppressive highly 
      active antiretroviral therapy (HAART). However, the low yield of cell isolates 
      has made this evaluation a difficult task. The present study analyzes whether 
      peripheral blood DCs from HIV-1-infected individuals on virally suppressive 
      HAART, with plasma HIV-1 RNA levels of less than 50 copies/ml, carry either HIV-1 
      provirus and/or HIV-1 virions. Peripheral blood DCs were isolated from a cohort 
      of 10 HIV-1-seropositive men taking suppressive HAART. In five patients, 
      plasmacytoid and myeloid dendritic cells were isolated to attempt to identify 
      their respective roles in HIV-1 residual disease. Viral out-growth assays were 
      performed in vitro, as well as gag and R/U5 polymerase chain reaction (PCR) 
      amplification of viral RNA and DNA, respectively, from DC and peripheral blood 
      mononuclear cell (PBMC) extracts. Fluorescence activated cell-sorting (FACS) data 
      revealed cellular yields from 85.90 to 95.18%, of relatively pure DCs isolated 
      from patients' PBMCs. Although HIV-1 RNA gag and DNA RU/5 were detected in all 
      PBMC samples isolated from the patients, proviral DNA and viral RNA forms were 
      not detected in any of the DC isolates. In addition, no replication-competent 
      virus was demonstrated in DC coculture assays, while virus was isolated from each 
      patients' CD8+ T-lymphocyte-depleted PBMC cocultures. Furthermore, HIV-1 gag 
      proviral DNA was not detected in either plasmacytoid or myeloid DC subfractions. 
      The current study suggests that in HIV-1-infected individuals treated with 
      suppressive HAART, peripheral blood DCs do not carry HIV-1 proviral DNA or viral 
      particles attached to their surface. These populations of peripheral blood DCs 
      are likely not a major HIV-1 reservoir in patients on HAART with clinically 
      undetectable plasma viral RNA.
FAU - Otero, Miguel
AU  - Otero M
AD  - The Dorrance H. Hamilton Laboratories, Center for Human Virology and Biodefense, 
      Division of Infectious Diseases and Environmental Medicine, Jefferson Medical 
      College, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA.
FAU - Nunnari, Giuseppe
AU  - Nunnari G
FAU - Leto, Daniela
AU  - Leto D
FAU - Sullivan, Julie
AU  - Sullivan J
FAU - Wang, Feng-Xiang
AU  - Wang FX
FAU - Frank, Ian
AU  - Frank I
FAU - Xu, Yan
AU  - Xu Y
FAU - Patel, Charvi
AU  - Patel C
FAU - Dornadula, Geethanjali
AU  - Dornadula G
FAU - Kulkosky, Joseph
AU  - Kulkosky J
FAU - Pomerantz, Roger J
AU  - Pomerantz RJ
LA  - eng
GR  - AI 45008/AI/NIAID NIH HHS/United States
GR  - AI146289/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - AIDS Res Hum Retroviruses
JT  - AIDS research and human retroviruses
JID - 8709376
RN  - 0 (Anti-HIV Agents)
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Anti-HIV Agents/therapeutic use
MH  - *Antiretroviral Therapy, Highly Active
MH  - CD8-Positive T-Lymphocytes/immunology/virology
MH  - Cohort Studies
MH  - DNA, Viral/blood
MH  - Dendritic Cells/*virology
MH  - HIV Infections/blood/drug therapy/immunology/*virology
MH  - HIV-1/drug effects/isolation & purification/*physiology
MH  - Humans
MH  - Leukocytes, Mononuclear/virology
EDAT- 2004/01/08 05:00
MHDA- 2004/04/13 05:00
CRDT- 2004/01/08 05:00
PHST- 2004/01/08 05:00 [pubmed]
PHST- 2004/04/13 05:00 [medline]
PHST- 2004/01/08 05:00 [entrez]
AID - 10.1089/088922203771881194 [doi]
PST - ppublish
SO  - AIDS Res Hum Retroviruses. 2003 Dec;19(12):1097-103. doi: 
      10.1089/088922203771881194.