PMID- 14709814
OWN - NLM
STAT- MEDLINE
DCOM- 20040413
LR  - 20190605
IS  - 0916-7250 (Print)
IS  - 0916-7250 (Linking)
VI  - 65
IP  - 12
DP  - 2003 Dec
TI  - Serum from dogs Infected with Babesia gibsoni inhibits maturation of 
      reticulocytes and erythrocyte 5'-nucleotidase activity in vitro.
PG  - 1281-6
AB  - Erythrocyte 5'-nucleotidase is thought to be involved in the maturation of 
      erythrocytes. In the present study, in vitro incubation of canine erythrocytes 
      demonstrated that significant inhibition of 5'-nucleotidase activity occurred in 
      the presence of serum from dogs infected with Babesia gibsoni, when the enzyme 
      was assayed with cytidine 5'-monophosphate (5'-CMP) and inosine 5'-monophosphate 
      (5'-IMP) as substrates. The multiplication of B. gibsoni in in vitro culture also 
      resulted in a significant decrease in the enzyme activity of erythrocytes in the 
      culture. Furthermore, the infected serum and 5'-CMP retarded the maturation of 
      canine reticulocytes in vitro. These results suggested that nucleotides such as 
      5'-CMP and 5'-IMP might accumulate in young erythrocytes and/or serum in dogs 
      infected with B. gibsoni as a result of decreased activity of erythrocyte 
      5'-nucleotidase, resulting in the delayed maturation of reticulocytes.
FAU - Hossain, Mohammad Alamgir
AU  - Hossain MA
AD  - Department of Veterinary Clinical Sciences, Graduate School of Veterinary 
      Medicine, Hokkaido University, Sapporo, Japan.
FAU - Yamato, Osamu
AU  - Yamato O
FAU - Yamasaki, Masahiro
AU  - Yamasaki M
FAU - Jeong, Ja-Ryong
AU  - Jeong JR
FAU - Chang, Hye-Sook
AU  - Chang HS
FAU - Maede, Yoshimitsu
AU  - Maede Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - J Vet Med Sci
JT  - The Journal of veterinary medical science
JID - 9105360
RN  - EC 3.1.3.5 (5'-Nucleotidase)
SB  - IM
MH  - 5'-Nucleotidase/antagonists & inhibitors/*blood
MH  - Animals
MH  - Babesia/*pathogenicity
MH  - Babesiosis/blood/*veterinary
MH  - Cellular Senescence/physiology
MH  - Dog Diseases/blood/*parasitology
MH  - Dogs
MH  - Erythrocytes/enzymology/*parasitology
MH  - Reticulocytes/*parasitology
EDAT- 2004/01/08 05:00
MHDA- 2004/04/14 05:00
CRDT- 2004/01/08 05:00
PHST- 2004/01/08 05:00 [pubmed]
PHST- 2004/04/14 05:00 [medline]
PHST- 2004/01/08 05:00 [entrez]
AID - 10.1292/jvms.65.1281 [doi]
PST - ppublish
SO  - J Vet Med Sci. 2003 Dec;65(12):1281-6. doi: 10.1292/jvms.65.1281.