PMID- 14710247
OWN - NLM
STAT- MEDLINE
DCOM- 20040430
LR  - 20171116
IS  - 1671-167X (Print)
IS  - 1671-167X (Linking)
VI  - 35
IP  - 6
DP  - 2003 Dec 18
TI  - [Ultrastructural and immunoelectron microscopic study on light chain deposition 
      diseases and primary amyloidosis of the kidney].
PG  - 576-80
AB  - OBJECTIVE: To investigate the role of electron microscopy (EM) and immunoelectron 
      microscopy (IEM) in the diagnosis of light chain deposition diseases (LCDD) and 
      primary amyloidosis (AL) of the kidney. METHODS Renal biopsy specimens collected 
      in our hospital from Jan. 1994 to Feb. 2002 were studied by EM, and 
      ultrastructural immunogold labeling for light chains (kappa, Lambda) were made on 
      the cases suggested of LCDD and early stage AL in the preliminary diagnosis. 
      RESULTS: Six cases were diagnosed as LCDD, and punctate, granular, electron-dense 
      material was identified in the outside of tubular basement membrane (TBM), inner 
      layer of glomerular basement membrane (GBM) and vascular walls by EM. Two of 
      which showed interrupted segmental localization of granular material in 
      subendothelial areas of GBM. The granular material was labeled by monotypical 
      light chains (4 cases of kappa chain, 2 cases of Lambda chain). Thirty-six cases 
      were diagnosed as AL. EM revealed fibrillary material distributed in glomerular 
      mesangium, GBM and vascular walls; 15 of them showed segmental distribution of 
      fibrils in mesangium and/or GBM, and were further studied by IEM labeling for 
      light chains. The fibrillary material was labeled by monotypical light chains (4 
      cases of kappa chain, 11 cases of Lambda chain). CONCLUSION The diagnosis of 
      renal LCDD and early stage of AL depended on EM, IEM is a unique diagnostic tool 
      in early stage of LCDD and AL.
FAU - Wang, Suxia
AU  - Wang S
AD  - Lab of Electron Microscopy, Peking University First Hospital, Beijing 100034, 
      China. sxwzp@sina.com
FAU - Zou, Wanzhong
AU  - Zou W
FAU - Wang, Mei
AU  - Wang M
FAU - Xie, Yanling
AU  - Xie Y
FAU - Chai, Lijun
AU  - Chai L
FAU - Tang, Xiuying
AU  - Tang X
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Beijing Da Xue Xue Bao Yi Xue Ban
JT  - Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health 
      sciences
JID - 101125284
RN  - 0 (Immunoglobulin Light Chains)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Amyloidosis/*pathology
MH  - Female
MH  - Humans
MH  - Immunoglobulin Light Chains/*metabolism
MH  - Kidney/*ultrastructure
MH  - Kidney Diseases/*pathology
MH  - Male
MH  - Microscopy, Immunoelectron
MH  - Middle Aged
EDAT- 2004/01/08 05:00
MHDA- 2004/05/01 05:00
CRDT- 2004/01/08 05:00
PHST- 2004/01/08 05:00 [pubmed]
PHST- 2004/05/01 05:00 [medline]
PHST- 2004/01/08 05:00 [entrez]
PST - ppublish
SO  - Beijing Da Xue Xue Bao Yi Xue Ban. 2003 Dec 18;35(6):576-80.