PMID- 14710441
OWN - NLM
STAT- MEDLINE
DCOM- 20040628
LR  - 20191026
IS  - 0190-2148 (Print)
IS  - 0190-2148 (Linking)
VI  - 29
IP  - 7
DP  - 2003 Oct-Nov
TI  - Heterogenous and compartment-specific activation of nonlymphocytic, mononuclear 
      cells in intraabdominal sepsis.
PG  - 503-21
AB  - Although sepsis is associated with increased production of cellular pro- and 
      anti-inflammatory mediators by monocyte/macrophages, the compartmentalization and 
      nature of such activation has not been clearly defined. The authors examined the 
      activation of nonlymphocytic mononuclear cells in different compartments in the 
      cecal ligation and puncture (CLP) model of septic peritonitis. Control and CLP 
      rat mononuclear cells from the peritoneal cavity, bronchoalveolar, as well as the 
      lung vascular compartment were isolated 24 and 48 hours post surgery and release 
      of nitric oxide (NO), interleukin (IL)-12, and monocyte chemoattractant protein-1 
      (MCP-1) was measured from culture media. Peritoneal macrophages (PMs) from CLP 
      rats increased release of all three mediators compared to controls. Cells from 
      the lung vascular compartment after CLP increased release of NO, but MCP-1 
      release was unchanged. Levels of IL-12 were not detectable. Similarly, 
      bronchoalveolar macrophages (BMs) of CLP rats had increased release of NO, 
      whereas IL-12 was not detectable. Release of MCP-1 increased 48 hours after CLP. 
      Almost all PMs and BMs possessed innate phagocytic ability that was not altered 
      during sepsis. The percentage of cells in the lung vascular compartment that had 
      phagocytic ability, increased 48 hours post CLP, versus controls. The authors 
      also evaluated lung injury at 24 hours after surgery by measurement of 
      bronchoalveolar lavage protein and LDH activity. There was an increase in both 
      these parameters 24 hours after CLP as compared to controls. Thus, there was 
      heterogenous and compartment-specific activation of mononuclear cells in sepsis. 
      There was nonspecific inflammatory activation in the primary site of injury. In a 
      remote organ (lung), the authors show for the first time that there was selective 
      activation of NO without increased release of the proinflammatory cytokine, 
      IL-12. Phagocytic activity was maintained in the bronchoalveolar compartment 
      whereas in the lung vascular compartment, the percentage of phagocytic cells 
      increased.
FAU - Wang, C
AU  - Wang C
AD  - Department of Cell Biology, University of Medicine and Dentistry, New Jersey 
      School of Osteopathic Medicine, Stratford, New Jersey, USA.
FAU - Kuzma, M
AU  - Kuzma M
FAU - Qiu, G
AU  - Qiu G
FAU - Yin, K
AU  - Yin K
LA  - eng
GR  - HL58172/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Exp Lung Res
JT  - Experimental lung research
JID - 8004944
RN  - 0 (Cytokines)
RN  - 31C4KY9ESH (Nitric Oxide)
SB  - IM
MH  - Animals
MH  - *Body Fluid Compartments
MH  - Bronchoalveolar Lavage Fluid/chemistry/cytology
MH  - Cecum/injuries/metabolism/pathology
MH  - Cells, Cultured
MH  - Cytokines/metabolism
MH  - Disease Models, Animal
MH  - Lung/blood supply/metabolism
MH  - *Macrophage Activation
MH  - Macrophages/classification/metabolism/*pathology
MH  - Male
MH  - Nitric Oxide/metabolism
MH  - Peritoneal Cavity
MH  - Peritonitis/complications/metabolism/*pathology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Sepsis/etiology/metabolism/*pathology
EDAT- 2004/01/09 05:00
MHDA- 2004/06/29 05:00
CRDT- 2004/01/09 05:00
PHST- 2004/01/09 05:00 [pubmed]
PHST- 2004/06/29 05:00 [medline]
PHST- 2004/01/09 05:00 [entrez]
AID - 10.1080/01902140303779 [doi]
PST - ppublish
SO  - Exp Lung Res. 2003 Oct-Nov;29(7):503-21. doi: 10.1080/01902140303779.