PMID- 14713022
OWN - NLM
STAT- MEDLINE
DCOM- 20040429
LR  - 20190917
IS  - 0730-7268 (Print)
IS  - 0730-7268 (Linking)
VI  - 22
IP  - 12
DP  - 2003 Dec
TI  - Combination of microautoradiography and fluorescence in situ hybridization for 
      identification of microorganisms degrading xenobiotic contaminants.
PG  - 2840-4
AB  - One of the central topics in environmental bioremediation research is to identify 
      microorganisms that are capable of degrading the contaminants of interest. Here 
      we report application of combined microautoradiography (MAR) and fluorescence in 
      situ hybridization (FISH). The method has previously been used in a number of 
      systems; however, here we demonstrate its feasibility in studying the degradation 
      of xenobiotic compounds. With a model system (coculture of Pseudomonas putida B2 
      and Sphingomonas stygia incubated with [14C] o-nitrophenol), combination of MAR 
      and FISH was shown to be able to successfully identify the microorganisms 
      degrading o-nitrophenol. Compared with the conventional techniques, MAR-FISH 
      allows fast and accurate identification of the microorganisms involved in 
      environmental contaminant degradation.
FAU - Yang, Yanru
AU  - Yang Y
AD  - ETH Zurich, Institute of Terrestrial Ecology, Soil Biology, Grabenstrasse 3, 8952 
      Schlieren, Switzerland.
FAU - Zarda, Annatina
AU  - Zarda A
FAU - Zeyer, Josef
AU  - Zeyer J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Environ Toxicol Chem
JT  - Environmental toxicology and chemistry
JID - 8308958
RN  - 0 (Xenobiotics)
SB  - IM
MH  - Autoradiography/methods
MH  - Biodegradation, Environmental
MH  - Environmental Monitoring/methods
MH  - In Situ Hybridization, Fluorescence
MH  - Pseudomonas putida/*genetics/*physiology
MH  - Sphingomonas/*genetics/*physiology
MH  - Xenobiotics/*metabolism
EDAT- 2004/01/10 05:00
MHDA- 2004/04/30 05:00
CRDT- 2004/01/10 05:00
PHST- 2004/01/10 05:00 [pubmed]
PHST- 2004/04/30 05:00 [medline]
PHST- 2004/01/10 05:00 [entrez]
AID - 10.1897/02-423 [doi]
PST - ppublish
SO  - Environ Toxicol Chem. 2003 Dec;22(12):2840-4. doi: 10.1897/02-423.