PMID- 14714273
OWN - NLM
STAT- MEDLINE
DCOM- 20040823
LR  - 20220408
IS  - 0947-7349 (Print)
IS  - 0947-7349 (Linking)
VI  - 111
IP  - 8
DP  - 2003 Dec
TI  - Vitamin D receptor gene polymorphisms and association with type 2 diabetes 
      mellitus in a Polish population.
PG  - 505-9
AB  - Vitamin D plays an important role in insulin secretion. There is also evidence 
      that this steroid may influence the insulin sensitivity. Thus genes involved in 
      its metabolic pathway have been regarded as good candidates for type 2 diabetes 
      mellitus (T2DM). One of them is vitamin D receptor gene (VDR). Its multiple 
      polymorphisms have been examined for the association with T2DM in several 
      populations. Those studies did not provide clear answers about the role of VDR in 
      this disease. The aim of the study was to search for the association of FokI, 
      ApaI, BsmI, and TaqI polymorphisms of VDR gene with T2DM in a Polish population 
      using a case-control study design. Overall, 548 individuals were examined: 308 
      T2DM patients and 240 control individuals. The study groups were genotyped for 
      VDR FokI, ApaI, BsmI, and TaqI variants using the restriction fragment length 
      polymorphism (RFLP) method. Since variants of ApaI, BsmI, and TaqI polymorphisms 
      were in very strong linkage disequilibrium, three loci haplotypes could be 
      assigned to phase-unknown individuals with a high degree of confidence. 
      Differences in allele, genotype, haplotype, and haplotype combination 
      distribution between the groups were examined by chi2 test. The VDR allele 
      frequencies for T2DM patients and controls were as follows: FokI-F/f - 53.4 
      %/46.6 % vs. 55.2 %/44.8 %, BsmI-B/b - 34.4 %/65.6 % vs. 37.5 %/62.5 %, ApaI-A/a 
      - 47.9 %/52.1 % vs 50.9 %/49.1 %, TaqI-T/t - 67.6 %/32.4 % vs. 62.7 %/37.3 %, 
      respectively. There was no difference between the groups in allele frequency. 
      Similarly, distribution of genotypes, three locus BsmI/ApaI/TaqI haplotypes and 
      their combinations were similar in the groups. In conclusion, our study did not 
      provide evidence for the association of four examined VDR polymorphisms with T2DM 
      in a Polish population. We postulate that to fully determine whether the sequence 
      differences in VDR gene are susceptibility variants for T2DM, additional studies 
      in different populations are required in a large study group.
FAU - Malecki, M T
AU  - Malecki MT
AD  - Department of Metabolic Diseases, Medical College, Jagiellonian University, 
      31-501 Krakow, Poland. mmalecki@cm-uj.krakow.pl
FAU - Frey, J
AU  - Frey J
FAU - Moczulski, D
AU  - Moczulski D
FAU - Klupa, T
AU  - Klupa T
FAU - Kozek, E
AU  - Kozek E
FAU - Sieradzki, J
AU  - Sieradzki J
LA  - eng
GR  - 1 R03 TW01351-01/TW/FIC NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Germany
TA  - Exp Clin Endocrinol Diabetes
JT  - Experimental and clinical endocrinology & diabetes : official journal, German 
      Society of Endocrinology [and] German Diabetes Association
JID - 9505926
RN  - 0 (Receptors, Calcitriol)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Alleles
MH  - Case-Control Studies
MH  - Diabetes Mellitus, Type 2/*genetics
MH  - Female
MH  - Gene Frequency
MH  - Genotype
MH  - Haplotypes
MH  - Humans
MH  - Linkage Disequilibrium
MH  - Male
MH  - Middle Aged
MH  - Poland
MH  - *Polymorphism, Genetic
MH  - Polymorphism, Restriction Fragment Length
MH  - Receptors, Calcitriol/*genetics
EDAT- 2004/01/10 05:00
MHDA- 2004/08/24 05:00
CRDT- 2004/01/10 05:00
PHST- 2004/01/10 05:00 [pubmed]
PHST- 2004/08/24 05:00 [medline]
PHST- 2004/01/10 05:00 [entrez]
AID - 10.1055/s-2003-44711 [doi]
PST - ppublish
SO  - Exp Clin Endocrinol Diabetes. 2003 Dec;111(8):505-9. doi: 10.1055/s-2003-44711.