PMID- 14714883
OWN - NLM
STAT- MEDLINE
DCOM- 20040804
LR  - 20191108
IS  - 1052-0295 (Print)
IS  - 1052-0295 (Linking)
VI  - 78
IP  - 3-4
DP  - 2003 Jun-Aug
TI  - Histological investigation of organisms with hard skeletons: a case study of 
      siliceous sponges.
PG  - 191-9
AB  - Siliceous and calcareous sponges commonly are treated with acid to remove the 
      spicules prior to embedding and cutting for histological investigations. 
      Histology of spiculated sponge tissue represents a challenging problem in sponge 
      histotechnology. Furthermore, fluorescence in situ hybridization (FISH), a key 
      method for studying sponge-associated microbes, is not possible after acid 
      treatment. For a broad range of siliceous sponge species, we developed and 
      evaluated methods for embedding in paraffin, methylmethacrylate resins, LR White 
      resin and cryomatrix. Different methods for cutting tissue blocks as well as 
      mounting and staining sections also were tested. Our aim was to enable 
      histological investigations and FISH without prior removal of the spicules. To 
      obtain an overview of tissue and skeleton arrangement, we recommend embedding 
      tissue blocks with LR White resin combined with en bloc staining techniques for 
      large specimens with thick and numerous spicules, but paraffin embedding and 
      subsequent staining for whole small specimens. For FISH on siliceous sponges, we 
      recommend Histocryl embedding if the spicule content is high, but paraffin 
      embedding if it is low. Classical histological techniques are used for detailed 
      tissue examinations.
FAU - Hoffmann, F
AU  - Hoffmann F
AD  - University of Gottingen, Department of Geosciences, Geobiology Group, 
      Goldschmidtstrasse 3, D-37077 Gottingen, Germany. fhoffma@gwdg.de
FAU - Janussen, D
AU  - Janussen D
FAU - Drose, W
AU  - Drose W
FAU - Arp, G
AU  - Arp G
FAU - Reitner, J
AU  - Reitner J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Biotech Histochem
JT  - Biotechnic & histochemistry : official publication of the Biological Stain 
      Commission
JID - 9107378
SB  - IM
MH  - Animals
MH  - In Situ Hybridization, Fluorescence
MH  - Paraffin Embedding
MH  - Porifera/*anatomy & histology/*cytology
MH  - Staining and Labeling
MH  - Tissue Embedding/*methods
MH  - Tissue Fixation
EDAT- 2004/01/13 05:00
MHDA- 2004/08/05 05:00
CRDT- 2004/01/13 05:00
PHST- 2004/01/13 05:00 [pubmed]
PHST- 2004/08/05 05:00 [medline]
PHST- 2004/01/13 05:00 [entrez]
AID - 10.1080/10520290310001613042 [doi]
PST - ppublish
SO  - Biotech Histochem. 2003 Jun-Aug;78(3-4):191-9. doi: 10.1080/10520290310001613042.