PMID- 14715556
OWN - NLM
STAT- MEDLINE
DCOM- 20040415
LR  - 20190509
IS  - 1071-412X (Print)
IS  - 1098-6588 (Electronic)
IS  - 1071-412X (Linking)
VI  - 11
IP  - 1
DP  - 2004 Jan
TI  - High prevalence of seropositivity to a major allergen of Anisakis simplex, Ani s 
      1, in dyspeptic patients.
PG  - 115-8
AB  - Finding evidence of anisakidosis requires invasive methods. We have developed a 
      serological assay based on the detection of an immunoglobulin E (IgE) 
      specifically directed against Ani s 1 protein, a major parasite allergen of 
      Anisakis simplex, which has shown a high level of accuracy in the diagnosis of 
      anisakidosis. We used this tool to determine the prevalence of anti-Ani s 1 IgE 
      in dyspeptic patients and to investigate if its seropositivity could be related 
      to epidemiological factors other than raw fish consumption. A total of 174 
      dyspeptic patients who submitted to upper digestive tract endoscopy were studied. 
      Specific IgE against Ani s 1 was determined by immunoblotting. Quantitative 
      information on smoking, alcohol consumption, and fish consumption as well as a 
      history of gastric surgery was recorded. Twenty-four (13.8%) patients were 
      seropositive for Ani s 1 protein. The seroprevalence of anti-Ani s 1 IgE 
      increased with age in patients who were less than 62 years old (P = 0.047). 
      Seropositivity to Ani s 1 was associated with the consumption of fish in vinegar 
      (P < 0.001), raw fish (P = 0.001), and smoked fish (P = 0.007). There was no 
      relationship between anti-Ani s 1 IgE seropositivity and the number of cigarettes 
      smoked (P = 0.098) or alcohol intake (P = 0.179). Five patients had undergone 
      previous gastric surgery, and three of those patients were seropositive for Ani s 
      1 (P = 0.019). In multivariate analysis, the consumption of fish in vinegar (P = 
      0.006), raw fish (P = 0.017), and smoked fish (P = 0.002) and a history of 
      gastric surgery (P = 0.005) were independent factors associated with anti-Ani s 1 
      IgE detection. In conclusion, at present, anisakidosis might frequently be 
      underdiagnosed, and it might have a clinical role in patients with upper 
      dyspeptic symptoms. Uncooked-fish ingestion and previous gastric surgery were 
      associated with seropositivity for Ani s 1 protein.
FAU - Toro, Carlos
AU  - Toro C
AD  - Services of Infectious Diseases, Hospital Carlos III and Department of Medicine, 
      European University of Madrid, Madrid, Spain. carlostororueda@hotmail.com
FAU - Caballero, Maria Luisa
AU  - Caballero ML
FAU - Baquero, Margarita
AU  - Baquero M
FAU - Garcia-Samaniego, Javier
AU  - Garcia-Samaniego J
FAU - Casado, Isabel
AU  - Casado I
FAU - Rubio, Margarita
AU  - Rubio M
FAU - Moneo, Ignacio
AU  - Moneo I
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Clin Diagn Lab Immunol
JT  - Clinical and diagnostic laboratory immunology
JID - 9421292
RN  - 0 (Allergens)
RN  - 0 (Antibodies, Helminth)
RN  - 0 (Antigens, Helminth)
RN  - 0 (Calcium-Binding Proteins)
RN  - 0 (Helminth Proteins)
RN  - 0 (troponin-like protein, Anisakis simplex)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Allergens/*immunology
MH  - Animals
MH  - Anisakiasis/*diagnosis/*immunology
MH  - Anisakis/*immunology
MH  - Antibodies, Helminth/*blood
MH  - Antigens, Helminth
MH  - Calcium-Binding Proteins/*immunology
MH  - Dyspepsia/*immunology/*parasitology
MH  - Fishes/parasitology
MH  - Food Parasitology
MH  - Helminth Proteins/*immunology
MH  - Humans
MH  - Immunoglobulin E/blood
PMC - PMC321343
EDAT- 2004/01/13 05:00
MHDA- 2004/04/16 05:00
PMCR- 2004/01/01
CRDT- 2004/01/13 05:00
PHST- 2004/01/13 05:00 [pubmed]
PHST- 2004/04/16 05:00 [medline]
PHST- 2004/01/13 05:00 [entrez]
PHST- 2004/01/01 00:00 [pmc-release]
AID - 0163 [pii]
AID - 10.1128/cdli.11.1.115-118.2004 [doi]
PST - ppublish
SO  - Clin Diagn Lab Immunol. 2004 Jan;11(1):115-8. doi: 
      10.1128/cdli.11.1.115-118.2004.