PMID- 14715744
OWN - NLM
STAT- MEDLINE
DCOM- 20040305
LR  - 20220309
IS  - 0095-1137 (Print)
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Linking)
VI  - 42
IP  - 1
DP  - 2004 Jan
TI  - Rapid diagnosis of human herpesvirus 6 infection by a novel DNA amplification 
      method, loop-mediated isothermal amplification.
PG  - 140-5
AB  - A novel nucleic acid amplification method, termed loop-mediated isothermal 
      amplification (LAMP), which amplifies DNA with high specificity, efficiency, and 
      rapidity under isothermal conditions, may be a valuable tool for the rapid 
      detection of infectious agents. LAMP was developed for human herpesvirus 6 
      (HHV-6), and its reliability was evaluated in this study. Although LAMP products 
      were detected in HHV-6 B and HHV-6 A DNA, they were not detected in HHV-7 and 
      human cytomegalovirus DNA. The sensitivity of the original HHV-6 LAMP protocol 
      was 50 copies/tube. In order to increase the method's sensitivity, HHV-6 LAMP was 
      modified by increasing the primer concentration. As a result of the modification, 
      sensitivity increased to 25 copies/tube. After these initial validation studies, 
      13 patients with fever were tested for HHV-6 by viral isolation, serological 
      analysis, and HHV-6 LAMP. In three of the eight patients with primary HHV-6 
      infection, HHV-6 DNA was detected in whole blood by the original HHV-6 LAMP 
      protocol in not only the acute phase but also the convalescent phase. HHV-6 DNA 
      was detected by modified HHV-6 LAMP in all eight plasma samples collected in the 
      acute phase; however, no HHV-6 DNA was detected in plasma samples collected in 
      the convalescent phase. Although HHV-6 DNA was detected in both the acute and 
      convalescent phases of whole-blood samples in patients with past HHV-6 infection, 
      it was not detected in plasma samples that did not contain latent viral DNA. 
      Thus, detection of HHV-6 DNA in plasma by using this modified HHV-6 LAMP protocol 
      is appropriate for diagnosis of active HHV-6 infection.
FAU - Ihira, Masaru
AU  - Ihira M
AD  - Department of Medical Information Technology, Fujita Health University College, 
      Konan, Aichi, Japan.
FAU - Yoshikawa, Tetsushi
AU  - Yoshikawa T
FAU - Enomoto, Yoshihiko
AU  - Enomoto Y
FAU - Akimoto, Shiho
AU  - Akimoto S
FAU - Ohashi, Masahiro
AU  - Ohashi M
FAU - Suga, Sadao
AU  - Suga S
FAU - Nishimura, Naoko
AU  - Nishimura N
FAU - Ozaki, Takao
AU  - Ozaki T
FAU - Nishiyama, Yukihiro
AU  - Nishiyama Y
FAU - Notomi, Tsugunori
AU  - Notomi T
FAU - Ohta, Yoshinori
AU  - Ohta Y
FAU - Asano, Yoshizo
AU  - Asano Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Base Sequence
MH  - DNA, Viral/analysis
MH  - Exanthema Subitum/*diagnosis
MH  - Herpesvirus 6, Human/*genetics
MH  - Molecular Sequence Data
MH  - Nucleic Acid Amplification Techniques/*methods
MH  - Polymerase Chain Reaction
MH  - Sensitivity and Specificity
PMC - PMC321673
EDAT- 2004/01/13 05:00
MHDA- 2004/03/06 05:00
PMCR- 2004/01/01
CRDT- 2004/01/13 05:00
PHST- 2004/01/13 05:00 [pubmed]
PHST- 2004/03/06 05:00 [medline]
PHST- 2004/01/13 05:00 [entrez]
PHST- 2004/01/01 00:00 [pmc-release]
AID - 0777 [pii]
AID - 10.1128/JCM.42.1.140-145.2004 [doi]
PST - ppublish
SO  - J Clin Microbiol. 2004 Jan;42(1):140-5. doi: 10.1128/JCM.42.1.140-145.2004.