PMID- 14722354
OWN - NLM
STAT- MEDLINE
DCOM- 20040330
LR  - 20210102
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 101
IP  - 4
DP  - 2004 Jan 27
TI  - T cell repertoire scanning is promoted by dynamic dendritic cell behavior and 
      random T cell motility in the lymph node.
PG  - 998-1003
AB  - Dendritic cells (DCs) ingest antigens in peripheral tissues and migrate to lymph 
      nodes where they present MHC class II-bound antigen to CD4(+) T cells. We used 
      two-photon microscopy to image the single-cell dynamics of interactions between 
      DCs and T cells within intact lymph nodes in the absence of relevant antigen. DCs 
      were fluorescently labeled in vivo by cutaneous injection of alum adjuvant 
      including carboxyfluorescein diacetate succinimidyl ester (CFSE). CFSE-positive 
      DCs (CD11c(+), CD11b(+), and low-to-intermediate CD8(+)) were observed in 
      draining lymph nodes 24-72 h later. Labeled DCs meandered slowly (2-3 microm x 
      min(-1)) in the T cell zone near B cell follicles but vigorously extended long 
      agile dendrites. Encounters between T cells and DCs arose as T cells moved 
      autonomously along random paths. Moreover, T cells did not accumulate around DCs, 
      and their relative velocities approaching and departing DCs were equivalent, 
      implying that T cells are not attracted toward DCs by chemotactic gradients but 
      rather encounter them by chance. T cell/DC contacts occurred primarily on 
      dendrites at arm's length from the DC soma and typically lasted approximately 3 
      min, enabling an individual DC to interact with up to 5000 T cells per hour. We 
      conclude that dynamic DC gesticulation and random T cell motility together 
      enhance the stochastic scanning of the T cell repertoire, thereby enabling rapid 
      initiation of the immune response.
FAU - Miller, Mark J
AU  - Miller MJ
AD  - Department of Physiology and Biophysics , University of California, Irvine, CA 
      92697, USA.
FAU - Hejazi, Arsalan S
AU  - Hejazi AS
FAU - Wei, Sindy H
AU  - Wei SH
FAU - Cahalan, Michael D
AU  - Cahalan MD
FAU - Parker, Ian
AU  - Parker I
LA  - eng
GR  - GM-41514/GM/NIGMS NIH HHS/United States
GR  - R01 GM048071/GM/NIGMS NIH HHS/United States
GR  - R37 GM048071/GM/NIGMS NIH HHS/United States
GR  - GM-48071/GM/NIGMS NIH HHS/United States
GR  - R01 GM041514/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
DEP - 20040113
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
SB  - IM
MH  - Animals
MH  - Cell Separation
MH  - Dendritic Cells/*immunology
MH  - Flow Cytometry
MH  - Lymph Nodes/*immunology
MH  - Mice
MH  - Mice, Transgenic
MH  - *T-Lymphocyte Subsets
PMC - PMC327133
EDAT- 2004/01/15 05:00
MHDA- 2004/03/31 05:00
PMCR- 2004/07/27
CRDT- 2004/01/15 05:00
PHST- 2004/01/15 05:00 [pubmed]
PHST- 2004/03/31 05:00 [medline]
PHST- 2004/01/15 05:00 [entrez]
PHST- 2004/07/27 00:00 [pmc-release]
AID - 0306407101 [pii]
AID - 1010998 [pii]
AID - 10.1073/pnas.0306407101 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 2004 Jan 27;101(4):998-1003. doi: 
      10.1073/pnas.0306407101. Epub 2004 Jan 13.