PMID- 14722715
OWN - NLM
STAT- MEDLINE
DCOM- 20040426
LR  - 20181130
IS  - 0001-6322 (Print)
IS  - 0001-6322 (Linking)
VI  - 107
IP  - 3
DP  - 2004 Mar
TI  - Infection of human astrocytes and glioblastoma cells with Toxoplasma gondii: 
      monocyte chemotactic protein-1 secretion and chemokine expression in vitro.
PG  - 245-9
AB  - In immunocompromised hosts, disruption of toxoplasmic cysts and conversion from 
      bradyzoites to tachyzoites occur in brain. In these areas, infiltrates of 
      mononuclear cells are observed. In the murine toxoplasmosis model, recent data 
      suggest that chemokines may play a role in leukocyte recruitment in the central 
      nervous system (CNS). This study analyzed the monocyte chemotactic protein-1 
      (MCP-1) secretion and chemokine expression after Toxoplasma gondii infection of 
      human astrocytes, glioblastoma cells (U373) and fibroblasts (MRC5) in vitro. T. 
      gondii infection of these CNS cells, astrocytes and glioblastoma cells 
      significantly increased MCP-1 secretion, particularly for astrocytes. In our 
      cellular models, the pattern of chemokine gene expression is dominated by MCP-1 
      expression. MCP-1 mRNAs were also quantified by real-time-PCR (LightCycler). The 
      behavior of cells studied after T. gondii infection was different (invasion and 
      growth) and the cell mechanisms of chemokine regulation could be dependent on the 
      type of cells infected, while MCP-1 may contribute to the cell recruitment during 
      human cerebral reactivation of T. gondii.
FAU - Brenier-Pinchart, Marie-Pierre
AU  - Brenier-Pinchart MP
AD  - Interactions Cellulaires Parasite-Hote, EA UJF 2940, Faculte de Medecine, 
      Universite J. Fourier, La Tronche, 38000 Grenoble, France. 
      MPBrenierPinchart@chu-grenoble.fr
FAU - Blanc-Gonnet, Emmanuelle
AU  - Blanc-Gonnet E
FAU - Marche, Patrice N
AU  - Marche PN
FAU - Berger, Francois
AU  - Berger F
FAU - Durand, Francois
AU  - Durand F
FAU - Ambroise-Thomas, Pierre
AU  - Ambroise-Thomas P
FAU - Pelloux, Herve
AU  - Pelloux H
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20040114
PL  - Germany
TA  - Acta Neuropathol
JT  - Acta neuropathologica
JID - 0412041
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokines)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Animals
MH  - Astrocytes/metabolism/*parasitology
MH  - Blotting, Northern/methods
MH  - Brain Neoplasms/pathology
MH  - Cell Count/methods
MH  - Cell Division/physiology
MH  - Cell Line, Tumor
MH  - Chemokine CCL2/*metabolism
MH  - Chemokines/genetics/*metabolism
MH  - Enzyme-Linked Immunosorbent Assay/methods
MH  - Fibroblasts/metabolism/parasitology
MH  - Glioblastoma/pathology
MH  - Humans
MH  - RNA, Messenger/biosynthesis
MH  - Reverse Transcriptase Polymerase Chain Reaction/methods
MH  - Time Factors
MH  - Toxoplasma/*physiology
EDAT- 2004/01/15 05:00
MHDA- 2004/04/27 05:00
CRDT- 2004/01/15 05:00
PHST- 2003/07/10 00:00 [received]
PHST- 2003/11/14 00:00 [revised]
PHST- 2003/11/20 00:00 [accepted]
PHST- 2004/01/15 05:00 [pubmed]
PHST- 2004/04/27 05:00 [medline]
PHST- 2004/01/15 05:00 [entrez]
AID - 10.1007/s00401-003-0804-0 [doi]
PST - ppublish
SO  - Acta Neuropathol. 2004 Mar;107(3):245-9. doi: 10.1007/s00401-003-0804-0. Epub 
      2004 Jan 14.