PMID- 14724150
OWN - NLM
STAT- MEDLINE
DCOM- 20040227
LR  - 20190501
IS  - 0017-5749 (Print)
IS  - 1458-3288 (Electronic)
IS  - 0017-5749 (Linking)
VI  - 53
IP  - 2
DP  - 2004 Feb
TI  - Mislocalisation of hephaestin, a multicopper ferroxidase involved in basolateral 
      intestinal iron transport, in the sex linked anaemia mouse.
PG  - 201-6
AB  - BACKGROUND: Hephaestin is a multicopper ferroxidase required for basolateral 
      transport of iron from enterocytes. Sex linked anaemia (sla) mice have a defect 
      in the release of iron from intestinal enterocytes into the circulation due to an 
      interstitial deletion in the hephaestin gene (heph). RESULTS: We have 
      demonstrated that hephaestin is primarily localised to a supranuclear compartment 
      in both intestinal enterocytes and in cultured cells. In normal intestinal 
      enterocytes, hephaestin was also present on the basolateral surface. In sla mice, 
      hephaestin was present only in the supranuclear compartment. In contrast, the 
      iron permease Ireg1 localised to the basolateral membrane in both control and sla 
      mice. CONCLUSION: We suggest that mislocalisation of hephaestin likely 
      contributes to the functional defect in sla intestinal epithelium.
FAU - Kuo, Y M
AU  - Kuo YM
AD  - Departments of Medicine and Pediatrics, University of California-San Francisco, 
      San Francisco, CA 94143-0794, USA.
FAU - Su, T
AU  - Su T
FAU - Chen, H
AU  - Chen H
FAU - Attieh, Z
AU  - Attieh Z
FAU - Syed, B A
AU  - Syed BA
FAU - McKie, A T
AU  - McKie AT
FAU - Anderson, G J
AU  - Anderson GJ
FAU - Gitschier, J
AU  - Gitschier J
FAU - Vulpe, C D
AU  - Vulpe CD
LA  - eng
GR  - R01 DK 047192/DK/NIDDK NIH HHS/United States
GR  - 5R01 DK 57800/DK/NIDDK NIH HHS/United States
GR  - R01 DK056376/DK/NIDDK NIH HHS/United States
GR  - K08 DK 2823/DK/NIDDK NIH HHS/United States
GR  - 5R01 DK 56376/DK/NIDDK NIH HHS/United States
GR  - R01 DK057800/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Gut
JT  - Gut
JID - 2985108R
RN  - 0 (HEPH protein, human)
RN  - 0 (Heph protein, mouse)
RN  - 0 (Membrane Proteins)
RN  - E1UOL152H7 (Iron)
SB  - IM
MH  - Anemia, Iron-Deficiency/genetics/*metabolism
MH  - Animals
MH  - Base Sequence
MH  - Biological Transport
MH  - Cell Membrane/chemistry
MH  - Cells, Cultured
MH  - *Duodenum
MH  - Enterocytes/chemistry
MH  - Humans
MH  - Intestinal Mucosa/*chemistry
MH  - Intracellular Fluid/chemistry
MH  - Iron/*metabolism
MH  - Membrane Proteins/*analysis/genetics
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Inbred Strains
MH  - Molecular Sequence Data
PMC - PMC1774920
EDAT- 2004/01/16 05:00
MHDA- 2004/02/28 05:00
PMCR- 2007/02/01
CRDT- 2004/01/16 05:00
PHST- 2004/01/16 05:00 [pubmed]
PHST- 2004/02/28 05:00 [medline]
PHST- 2004/01/16 05:00 [entrez]
PHST- 2007/02/01 00:00 [pmc-release]
AID - 0530201 [pii]
AID - 10.1136/gut.2003.019026 [doi]
PST - ppublish
SO  - Gut. 2004 Feb;53(2):201-6. doi: 10.1136/gut.2003.019026.