PMID- 14725996
OWN - NLM
STAT- MEDLINE
DCOM- 20050224
LR  - 20190917
IS  - 0009-3084 (Print)
IS  - 0009-3084 (Linking)
VI  - 127
IP  - 2
DP  - 2004 Feb
TI  - Effect of phytosterols and phytostanols on the solubilization of cholesterol by 
      dietary mixed micelles: an in vitro study.
PG  - 121-41
AB  - The effect of a plant sterol, beta-sitosterol (SI), and a plant stanol, 
      sitostanol (SS), on the solubilization of cholesterol (CH) by model dietary mixed 
      micelles was examined under in vitro conditions with the use of gas 
      chromatography, isothermal titration calorimetry, NMR spectroscopy and cryogenic 
      transmission electron microscopy techniques. Free SI and SS were shown to reduce 
      the concentration of CH in dietary mixed micelles via a dynamic competition 
      mechanism. CH, SI and SS affect the microstructure of lipid vesicles and 
      influence the process of amphiphilic self-assembly of nutrients in the gut with 
      the formation of dietary mixed micelles in a similar manner. Therefore, 
      substitution of CH by phytosterols and phytostanols in the diet does not lead to 
      the notable changes in the mechanism of dietary mixed micelle formation and does 
      not affect the process of the intestinal transport of nutrients and drugs via the 
      micellar diffusion mechanism. Our experimental findings demonstrate that the 
      introduction of plant sterols and plant stanols into the diet is clearly 
      beneficial for the reduction of the intestinal uptake of cholesterol. Due to the 
      limited capacity of dietary mixed micelles to embody hydrophobic sterol/stanol 
      molecules, the micellar concentration of cholesterol is reduced and hence, its 
      transport towards the intestinal brush border membrane decreases.
FAU - Mel'nikov, Sergey M
AU  - Mel'nikov SM
AD  - Foods Research Centre, Unilever R&D Vlaardingen, Olivier van Noortlaan 120, 3133 
      AT Vlaardingen, The Netherlands. sergey.melnikov@unilever.com
FAU - Seijen ten Hoorn, Jack W M
AU  - Seijen ten Hoorn JW
FAU - Eijkelenboom, Astrid P A M
AU  - Eijkelenboom AP
LA  - eng
PT  - Journal Article
PL  - Ireland
TA  - Chem Phys Lipids
JT  - Chemistry and physics of lipids
JID - 0067206
RN  - 0 (Bile Acids and Salts)
RN  - 0 (Cholesterol, Dietary)
RN  - 0 (Hypolipidemic Agents)
RN  - 0 (Micelles)
RN  - 0 (Phosphatidylcholines)
RN  - 0 (Sitosterols)
RN  - 2UMI9U37CP (Oleic Acid)
RN  - 5LI01C78DD (gamma-sitosterol)
RN  - 97C5T2UQ7J (Cholesterol)
RN  - C2NJ9WO6O7 (stigmastanol)
SB  - IM
MH  - Bile Acids and Salts/chemistry
MH  - Calorimetry/methods
MH  - Cholesterol/*chemistry
MH  - Cholesterol, Dietary/pharmacokinetics
MH  - Chromatography, Gas
MH  - Cryoelectron Microscopy
MH  - Hypolipidemic Agents/pharmacokinetics/pharmacology
MH  - *Micelles
MH  - Nuclear Magnetic Resonance, Biomolecular
MH  - Oleic Acid/chemistry
MH  - Phosphatidylcholines/chemistry
MH  - Sitosterols/*chemistry/pharmacokinetics/pharmacology
MH  - Solubility
MH  - Thermodynamics
EDAT- 2004/01/17 05:00
MHDA- 2005/02/25 09:00
CRDT- 2004/01/17 05:00
PHST- 2004/01/17 05:00 [pubmed]
PHST- 2005/02/25 09:00 [medline]
PHST- 2004/01/17 05:00 [entrez]
AID - S0009308403001610 [pii]
AID - 10.1016/j.chemphyslip.2003.09.015 [doi]
PST - ppublish
SO  - Chem Phys Lipids. 2004 Feb;127(2):121-41. doi: 10.1016/j.chemphyslip.2003.09.015.